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Application of Necrostatin-1 as neutrophile granulocyte apoptosis accelerant

A technology of neutrophils and promoters, which is applied in the application field of neutrophil apoptosis promoters, and can solve the problems of no antioxidant effect and the like

Inactive Publication Date: 2015-11-25
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies have found that it has no inhibitory effect on apoptosis and autophagy, has no direct antioxidant effect, and has no significant effect on the morphology, proliferation, adhesion, and levels of ATP, calcium, and oxygen free radicals of normal cells.

Method used

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  • Application of Necrostatin-1 as neutrophile granulocyte apoptosis accelerant
  • Application of Necrostatin-1 as neutrophile granulocyte apoptosis accelerant
  • Application of Necrostatin-1 as neutrophile granulocyte apoptosis accelerant

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Embodiment Construction

[0027] For the convenience of description, the compound 5-(1H-indol-3-ylmethyl)-3-methyl-2-thione-4-imidazolidinone (Necrostatin-1) is abbreviated as Nec-1 below.

[0028] The technical solution of the present invention will be further described below in combination with experiments.

[0029] Experiment 1. Nec-1 promotes apoptosis of human neutrophils

[0030] 1. Experimental materials

[0031] 1.1 Neutrophils

[0032] The Third Affiliated Hospital of Southern Medical University recruited 30 healthy volunteers, informed the volunteers of the experimental content, and signed an informed consent form. The ethical protocol was reviewed and approved by the Ethics Committee of Southern Medical University. Take 10ml of peripheral blood from volunteers and separate neutrophils.

[0033] Test drug

[0034] Nec-1 was dissolved by adding DMSO, prepared into a solution with a concentration of 10 mg / ml, filtered and sterilized through a 0.22 mm microporous membrane, and stored at -20°...

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Abstract

The invention discloses an application of Necrostatin-1 as a neutrophile granulocyte apoptosis accelerant. Study on compound 5-(1H-benzpyrole-3-ylmethyl)-3-methyl-2-thioketone-4thioketone conducted by the inventor shows that except for the known function of inhibiting programmed necrosis, 5-(1H-benzpyrole-3-ylmethyl)-3-methyl-2-thioketone-4thioketone also has the effects of promoting the apoptosis of the neutrophile granulocyte, and treating neutrophile granulocyte apoptosis abnormity diseases by promoting the apoptosis of the neutrophile granulocyte, especially the acute lung injury caused by LPS. Molecules of 5-(1H-benzpyrole-3-ylmethyl)-3-methyl-2-thioketone-4thioketone are small, can be easily accepted by the mechanism, also can specifically act on neutrophile granulocyte to promote the apoptosis of the neutrophile granulocyte, and have no influences on the apoptosis of other cells.

Description

technical field [0001] The present invention relates to the new application of the compound, in particular to the application of Necrostatin-1 as neutrophil apoptosis accelerator. Background technique [0002] Neutrophils (polymorphonuclearneutrophil, PMN) are the shortest-lived cells in blood cells. They are terminally differentiated cells that differentiate and mature in the red bone marrow, and then released into the blood circulation. The average half-life in peripheral blood is 4- 10 hours, although its survival time is relatively short, it plays a very important role in the inflammatory response. On the one hand, it is the first line of defense of the body's immune defense system. On the other hand, if it is overactivated in the inflammatory focus , Delayed clearing or secondary necrosis, can aggravate the inflammatory response and surrounding tissue damage by releasing oxygen free radicals, proteolytic enzymes and other harmful contents, and even make the inflammation...

Claims

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Application Information

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IPC IPC(8): A61K31/4178A61P11/00A61P19/02A61P29/00A61P31/00A61P9/10
Inventor 孙尔维接红宇何懿周庆游黄学婵劳小斌谭礼平韩艳萍李杏
Owner SOUTHERN MEDICAL UNIVERSITY
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