Straw returning cellulose-decomposing biological control fungus, microbe and application of microbe

A fiber decomposition and biological control technology, applied in the field of microorganisms, can solve the problems of affecting soil tillage seed germination and seedling growth, slow degradation of straw, and unavailability of nutrients, achieving good application value, unsatisfactory solution effect, strong The effect of decomposition ability

Inactive Publication Date: 2015-11-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the traditional straw in situ returning technology, the degradation rate of straw is slow, and the nutrients in the straw cannot be fully utilized by the crops of the season, which affects soil cultivation, seed germination and crop seedling growth, and also causes some insect pests and pathogenic bacteria to grow in the soil. Survival for a long time, causing potential harm to crops, thus limiting its popularization and application

Method used

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  • Straw returning cellulose-decomposing biological control fungus, microbe and application of microbe
  • Straw returning cellulose-decomposing biological control fungus, microbe and application of microbe
  • Straw returning cellulose-decomposing biological control fungus, microbe and application of microbe

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment one, the screening of Trichoderma viridans F7JX993849

[0024] 1. Isolation and purification of strains

[0025] Collect 5 g of rice straw returning soil samples, place them in shake flasks containing 100 mL of enrichment medium, cultivate at a temperature of 30° C., and incubate at a constant temperature with a shaker speed of 180 rpm. Every 4 days, 10% of the inoculum was added to another enriched medium, and the acclimatization was repeated 5 times. The enrichment medium uses rice straw powder as the sole carbon source.

[0026] Take 150 μL of the enriched culture solution after the above training and spread it on the sodium carboxymethylcellulose-Congo red medium, and culture at 30°C for 4 days. The bacteria with a larger diameter of the hydrolysis circle were picked and transferred to an inorganic salt medium with sodium carboxymethyl cellulose as the sole carbon source, and continuously subcultured for more than 10 generations. The samples with good ...

Embodiment 2

[0033] Example 2, determination of the decomposing effect of Trichoderma viridans F7JX993849 on straw

[0034] 2.1 Preparation of Trichoderma viridans F7JX993849 bacterial suspension:

[0035] Trichoderma viridans F7JX993849 bacterial strain that will activate is inoculated in the carboxymethylcellulose sodium (CMC-Na) liquid medium of sterilizing, and the component of this liquid medium is: CMC-Na2.0g, yeast extract 1.0g, (NH 4 ) 2 SO 4 0.4g, KH 2 PO 4 0.25g, peptone 0.1g, MgSO 4 ·7H 2 O0.01g, distilled water to a volume of 1000mL, pH 7.0, 28°C, 180rpm, shaking culture for 24h, to make a bacterial suspension that is F7JX993849 bacterial suspension.

[0036] 2.2 Preparation of crude enzyme solution:

[0037]The F7JX993849 bacterial suspension prepared in 2.1 was inoculated into the rice straw medium according to the inoculum amount of 5%. The components of the rice straw medium are: 5 g of rice straw dry powder, 1.0 g of yeast extract, (NH 4 ) 2 SO 4 0.4g, KH 2 PO...

Embodiment 4

[0045] Embodiment four, bacterial strain antagonism to plant pathogenic bacteria

[0046] The invention adopts a two-point confrontation culture method to measure the antagonism effect of Trichoderma viridans F7JX993849 on plant pathogenic bacteria. Inoculate Trichoderma viridans F7JX993849 and 4 kinds of plant pathogens (Phytophthoracapsici of capsicum; Botrytiscinerea of ​​tomato; Didymellabryoniae of watermelon blight; Valsaceratosperma of apple rot) on the PDA plate respectively. The Experimental Center of the School of Resources and Environment is isolated and preserved. The plant pathogenic bacteria in the present invention are not limited to the above-mentioned specific strains, and other specific strains disclosed in the prior art can also be selected.

[0047] After cultivating Trichoderma viridans F7JX993849 and the above four plant pathogenic bacteria for 3 days under dark conditions at 28°C, use a sterilized puncher with a diameter of 5 mm to punch out the plates a...

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Abstract

The invention provides a straw returning cellulose-decomposing biological control fungus, a microbe and application of the microbe, wherein the strain F7JX993849 is classified and named as trichoderma virens which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 3.17613. The trichoderma virens F7JX993849 is relatively strong in decomposing capacity simultaneously on cellulose, hemicellulose and pectin components in crop straw. In addition, the trichoderma virens F7JX993849 is capable of antagonizing the invasion of phytopathogenic fungi, and has a biological control function. Furthermore, the microbe prepared from the straw returning cellulose-decomposing biological control fungus disclosed by the invention can be used for solving the problems of a general straw decomposition agent which is un-ideal in effect and is instable in the process of application; and the microbe has a good application value.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a fiber-decomposing biocontrol fungus for straw returning to the field, a bacterial agent and its application. Background technique [0002] my country's crop straw nutrient resources are very rich. Using straw returning technology to convert crop straw into high-quality organic fertilizer can realize a virtuous cycle of substances in the farmland ecosystem and can effectively solve soil nutrient depletion and fertility decline caused by repeated farming. It is an important measure to realize the sustainable development of agriculture and the construction of rural ecological environment in my country. However, in the traditional straw in situ returning technology, the degradation rate of straw is slow, and the nutrients in the straw cannot be fully utilized by the crops of the season, which affects soil cultivation, seed germination and crop seedling growth, and also caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C05F11/00C12R1/885
Inventor 宋宁宁王凯荣刘君
Owner QINGDAO AGRI UNIV
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