Method and fermentation medium for producing milbemycins through streptomyces fermentation

A fermentation medium and mirbemycin technology, applied in the method and the fermentation medium thereof, in the field of Streptomyces fermentative production of mirbemycin, can solve the problems of long time consumption, low success rate and high cost, and achieve the improvement of fermentation level, The effect of reducing production cost and increasing output

Active Publication Date: 2015-11-25
浙江大战生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such a change approach is technically difficult, costly, time-consuming, and has a low success rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for producing milbemycin by streptomyces fermentation comprises the steps:

[0027] (1) Slant surface activation culture: take the preserved strain (Streptomyces bingchengensis CGMCCNO.1734), inoculate it into the slant medium, place it in an incubator at 28°C with a relative humidity of 40%, and cultivate it for 9 days to obtain activation strains.

[0028] The slant medium is made by mixing the following raw materials in terms of mass percentage: 0.3% sucrose, 0.1% skimmed milk powder, 0.15% yeast extract (commercially available), 0.4% malt extract (commercially available), 2.5% agar, water Quantity, pH7.2±0.1, sterilized at 121°C for 20 minutes, and made into a slope.

[0029] (2) Seed expansion culture: take seeds from the slant medium, use a sterile inoculation loop to scrape the spores from the slant medium under aseptic conditions to make a spore suspension to a concentration of 10 7 spores / mL, then take 2mL of spore suspension and inoculate it into ...

Embodiment 2

[0036] The fermentation culture method that Streptomyces fermentation produces milbemycin comprises the steps:

[0037] (1) Inclined surface activation culture: take the preserved strain (Streptomyces bingchengensis CGMCCNO.1734), inoculate it into the slanted medium, place it in an incubator at 28°C with a relative humidity of 60%, and cultivate it for 8 days to obtain activation strains.

[0038] The slant medium is made by mixing the following raw materials in terms of mass percentage: 0.3% sucrose, 0.1% skimmed milk powder, 0.15% yeast extract (commercially available), 0.4% malt extract (commercially available), 2.5% agar, water Quantity, pH7.2±0.1, sterilized at 121°C for 20 minutes, and made into a slope.

[0039] (2) Seed expansion culture: take seeds from the slant medium, use a sterile inoculation loop to scrape the spores from the slant medium under aseptic conditions to make a spore suspension to a concentration of 10 8 spores / mL, then take 2mL of spore suspension...

Embodiment 3

[0046] The fermentation culture method that Streptomyces fermentation produces milbemycin comprises the steps:

[0047] (1) Slant surface activation culture: take preserved strains (Streptomyces milbei (DSM41911) purchased from DSMZ Collection Center), inoculate them into the slant medium, and place them in an incubator at 28°C after inoculation, with a relative humidity of 40-60°C. %, cultivated for 8-9d to obtain activated strains.

[0048] The slant medium is made by mixing the following raw materials in terms of mass percentage: 0.3% sucrose, 0.1% skimmed milk powder, 0.15% yeast extract (commercially available), 0.4% malt extract (commercially available), 2.5% agar, water Quantity, pH7.2±0.1, sterilized at 121°C for 20 minutes, and made into a slope.

[0049] (2) Seed expansion culture: take seeds from the slant medium, use a sterile inoculation loop to scrape the spores from the slant medium under aseptic conditions to make a spore suspension to a concentration of 10 7...

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PUM

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Abstract

The invention discloses a method and a fermentation medium for producing milbemycins through streptomyces fermentation. The fermentation level of milbemycins can be improved by improving the medium and fermentation process, so as to significantly improve the yield of the milbemycins and to reduce production cost; and the method is simple and easy to implement, and is applicable to industrial production.

Description

technical field [0001] The invention relates to the technical field of milbemycin production, in particular to a method for producing milbemycin by Streptomyces fermentation and a fermentation medium thereof. Background technique [0002] Milbemycins are macrolide antibiotics produced by Streptomyces, including a series of sixteen-membered ring components with very similar structures. Milbemycin has strong insecticidal, acaricidal, anthelmintic and anti-tumor biological activities, most of which have broad-spectrum control activities against agricultural pests, and is used to control such as aphids, mites, yellow-brown tent caterpillars, and intestinal parasites. Insects and other parasites that harm crops and livestock. Milbemycin has the characteristics of strong action, less dosage, safe and non-toxic to humans, does not pollute the environment, only kills insects and does not kill natural enemies of insects, etc. It can be used as a compound and rotation drug to preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/46
Inventor 徐海斌朱廷恒李春
Owner 浙江大战生物科技有限公司
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