Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting mitochondrial genomes and primer

A mitochondrial genome and primer pair technology, which can be used in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., and can solve problems such as inapplicability

Inactive Publication Date: 2015-11-25
BGI SHENZHEN CO LTD
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhang Maolei et al published the mitochondrial DNA detection method of exfoliated cells in the oral cavity. The samples containing nuclear DNA were detected. In forensic identification, keratinized tissue samples such as hair and nails are more common, and the nuclear DNA is rarely or even completely degraded. May not be suitable for testing such samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting mitochondrial genomes and primer
  • Method for detecting mitochondrial genomes and primer
  • Method for detecting mitochondrial genomes and primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1. Detection of hair mitochondrial genome without hair follicles based on next-generation sequencing

[0068] 1. Genomic DNA extraction

[0069] Take a hair about 5 cm long without a follicle (only keratinized tissue, that is, the hair shaft) as an example.

[0070] Prepare dithiothreitol (DTT) extract: Dissolve 1g of DTT in 6.66mL of ultrapure water. After fully dissolved, aliquot and store at -20°C for later use. use DNAInvestigator kit and dithiothreitol (DTT) extraction solution were used to extract genomic DNA from hair samples, as detailed below:

[0071] Digestion of hair samples: Add 300 μL of BufferATL, 20 μL of proteinase K and 20 μL of DTT, vortex and mix for 10 seconds, then place in a constant temperature mixer and shake at 56°C for 1 hour at 900 rpm.

[0072] Finally, 30 μL of the DNA sample was dissolved, and the concentration was determined with Qubit2.0. The concentration of the hair sample was 4.34 ng / μL, and stored at -20°C for later use....

Embodiment 2

[0149] Example 2. Detecting the Mitochondrial Genome of Nail Based on Next Generation Sequencing Technology

[0150] 1. Genomic DNA extraction: using the method in Example 1, extract the genomic DNA of a nail with a length of about 1 cm and a width of about 0.2 cm;

[0151] Prepare dithiothreitol (DTT) extract: Dissolve 1g of DTT in 6.66mL of ultrapure water. After fully dissolved, aliquot and store at -20°C for later use. use DNAInvestigator kit and dithiothreitol (DTT) extraction solution were used to extract genomic DNA from nail samples, as detailed below:

[0152] Digestion of nail samples: Add 300 μL of BufferATL, 20 μL of proteinase K and 20 μL of DTT, vortex and mix for 10 seconds, then place in a constant temperature mixer, shake at 56°C at 900 rpm for 2.5 hours.

[0153] The concentration of the nail sample was 8.16ng / μL, and it was stored at -20°C for future use.

[0154] 2. Sample preparation for sequencing

[0155] 1. Design the primers for amplifying mitoch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting mitochondrial genomes and a primer. A primer group for forensically medically identifying examined materials comprises four primer pairs. The four primer pairs respectively include a primer pair 1, a primer pair 2, a primer pair 3 and a primer pair 4, the primer pair 1 comprises an Mit1F primer and an Mit1R primer, the primer pair 2 comprises an Mit2F primer and an Mit2R primer, the primer pair 3 comprises an Mit3F primer and an Mit3R primer, and the primer pair 4 comprises an Mit4F primer and an Mit4R primer. The method and the primer have the advantages that full-length sequences of mitochondrial DNA (deoxyribonucleic acid) of samples with few genomes can be detected by the aid of the method, and accordingly important detection technologies can be provided for detecting trace examined materials in forensic medicine.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mitochondrial genome detection method and primers. Background technique [0002] With the rapid development of science and technology, the role of DNA testing technology in the field of forensic science is becoming more and more important. In 1985, Professor A.J. Jeffreys, a British geneticist, used DNA fingerprinting to help identify a rape criminal in a certain place, solved the case, opened the application of DNA testing technology in the field of forensic medicine, and greatly promoted the development of forensic science. Since then, DNA testing technology has been widely used in the field of forensic science, such as the identification of residue evidence at the murder scene, identification of the source of corpses, paternity identification, gender identification, and the establishment of criminal genetic fingerprint databases. [0003] In order to provide accurate and reliab...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
Inventor 崔路漫叶睿毕腾腾
Owner BGI SHENZHEN CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com