Optical excitation and detection system of fluorescent quantitative PCR detector

Abstract

The invention discloses an optical excitation and detection system of a fluorescent quantitative PCR detector, and belongs to a field of biological and medical science detection. The system provided by the invention comprises an optical excitation unit and an optical detection unit, wherein the optical excitation unit comprises several light sources, an excitation light optical filter and a holophote; the optical detection unit comprises several light splitting pieces, optical filters, imaging sensors and detection circuits. Light emitted from the light sources is formed into excitation light of a specific wavelength by the excitation light optical filter, the excitation light is reflected by the holophote and enters into a PCR test tube, fluorescent substances in an excitation light irradiation agent emit light, a part of the light enters into the optical detection unit from a hole in the side surface of a reaction tank, and finally the light is split and filtered by the optical detection unit and is imaged on the imaging sensors.

Description

technical field [0001] The invention relates to an optical excitation and detection system of a fluorescent quantitative PCR detector, which belongs to the field of biological and medical detection. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. Real-time fluorescent quantitative PCR technology refers to the method of adding fluorescent groups to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally performing quantitative analysis of unknown templates through standard curves. [0003] At present, among the fluorescent quantitative PCR detectors on the market, the biggest difference between each product lies in the optical excitation and detection modules. The optical excitation and detection modules of these products mainly have the following deficiencies: [0004] 1. Some fluorescent quantitative PCR detector...

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Problems solved by technology

[0005] 2. The optical path design of some fluorescence quantitative PCR detectors is very complicated, which increases the volume and cost of the product and reduces the reliability, such as the TOpticalThermocycler fluorescence quantitative PCR instrument of Biometra;

[0006] 3. Some fluorescent quantitative PCR detectors achieve the purpose of multi-light path detection by adding rotatable excitation light filter color wheels and emission light filter color wheels in the light path. The existence of these mechanical devices increases power consumption and reduces reliability

[0007] 4. In addition, most of the fluorescent quantitative PCR detectors currently on the market are medium and large machines used for laboratory testing. The optical excitation and detection modules are complex in structure, expensive, and poor in portability.

Users must take the prepared samples to the laboratory for testing, which affects the rapid diagnosis of some sudden diseases

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