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Haemophilus parasuis strain

A technology of Haemophilus suis and strains, applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems of difficulty in the prevention and treatment of Haemophilus parasuis, weak interactive immunity, and difficulty in achieving the expected immune effect of vaccines , to achieve a good effect of safety and prevention ability

Active Publication Date: 2015-12-02
LONGYAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the mutual immunity between the various Haemophilus parasuis serogroups is not strong. Prevention and treatment pose certain difficulties
[0004] At present, the domestic vaccines used for the immunization and prevention of Haemophilus parasuis disease include the inactivated Haemophilus parasuis disease vaccine (Z-1517 strain) produced by Boehringer Ingelheim Animal Health (USA) Co., Ltd. Haemophilus parasuis type 1 and 6 inactivated vaccines produced by pharmaceutical factories, Haemophilus parasuis type 4 and 6 produced by Huazhong Agricultural University, Wuhan Keqian Animal Biological Products Co., Ltd. Type 5 inactivated vaccines; the application of these vaccines has played a certain preventive role, but the pathogenic strains of the disease and multiple serotype infections have made it difficult for existing vaccines to achieve the expected immune effect. Therefore, it is used to participate in the preparation of prevention and treatment The suitable strain of Haemophilus parasuis disease vaccine caused by multiple serotypes of Haemophilus parasuis is an urgent problem for practitioners

Method used

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  • Haemophilus parasuis strain
  • Haemophilus parasuis strain
  • Haemophilus parasuis strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Isolation and Identification of Example 1 Bacterial Strain LYC2

[0014] 1. Isolation of strain LYC2:

[0015] In 2012, the applicant collected the lungs, joint effusion, and trachea of ​​piglets suspected of having Hps from a pig farm in Xinluo, Quanzhou City, Fujian Province under aseptic conditions, and inoculated them on TSA medium, and cultured them at a constant temperature of 37°C. In the box, the candle jar was anaerobically cultured for 24-48h, and the bacterial strain obtained from the cultivation was named strain LYC2.

[0016] 2. Identification of strain LYC2:

[0017] Initial identification:

[0018] The morphological and biochemical characteristics of the obtained strain LYC2 were determined, and the results are as follows: Gram-negative, small bacillus, with a variety of different forms, from single cocci to long, slender, and even filamentous No hemolysis, the isolate does not produce indole, can ferment glucose, fructose, sucrose, but not xylose, L-a...

Embodiment 2

[0028] Isolation and Identification of Example 2 Bacterial Strain LYD1

[0029] 1. Isolation of strain LYD1:

[0030] In 2009, the applicant collected the lungs, joint effusion, and trachea of ​​piglets suspected of having Hps from a pig farm in Xinluo, Quanzhou City, Fujian Province under aseptic conditions, and inoculated them on TSA medium, and cultured them at a constant temperature of 37°C. In the box, candle jars were anaerobically cultured for 24-48 hours, and the strain obtained from the culture was named strain LYD1.

[0031] 2. Identification of strain LYD1:

[0032] Initial identification:

[0033] The morphological and biochemical characteristics of the obtained strain LYD1 were determined, and the results were consistent with the preliminary identification results in Example 1; therefore, it can be preliminarily determined that the strain LYD1 also belongs to the genus Haemophilus parasuis.

[0034] Further identification:

[0035] Extract template: refer to t...

Embodiment 3

[0042] The isolation and identification of embodiment 3 bacterial strain LYH5

[0043] 1. Isolation of strain LYH5:

[0044] In 2011, the applicant collected the lungs, joint effusion, and trachea of ​​piglets suspected of having Hps in a pig farm in Yongding, Longyan City, Fujian Province under aseptic conditions, and inoculated them on TSA medium, and placed them in a constant temperature incubator at 37°C. In the process of anaerobic cultivation in candle jars for 24-48 hours, the strain obtained from the cultivation was named strain LYH5

[0045] 2. Identification of strain LYH5:

[0046] Initial identification:

[0047] The morphological and biochemical characteristics of the obtained strain LYH5 were determined, and the results were consistent with the preliminary identification results in Example 1; therefore, it can be preliminarily determined that the strain LYH5 also belongs to the genus Haemophilus parasuis.

[0048] Further identification:

[0049] Extraction t...

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Abstract

The invention provides a Haemophilus parasuis strain LYC2. The strain is collected at the China General Microbiological Culture Collection Center on 21st, July, 2015, in No. 3 courtyard No.1 on the West Beichen Road in the Chaoyang District of Beijing and under serial number of CGMCC No.11147. The strain LYC2 can be used for participating in preparing vaccines for preventing and treating diseases caused by Haemophilus parasuis of various serotypes, and the vaccines prepared finally are high in both safety and preventing and treating capability.

Description

【Technical field】 [0001] The invention belongs to the field of biology, and in particular relates to a strain of Haemophilus parasuis. 【Background technique】 [0002] Haemophilus parasuis was once considered to be a conditional and sporadic disease of piglets caused by stress. With the improvement of industrialization and intensification of pig farming, it has become the most serious bacterial disease that harms piglets and growing pigs. disease. [0003] Haemophilus parasuis mainly harms piglets and growing pigs aged 2 to 28 weeks, and weaned piglets aged 5 to 8 weeks are the most serious, causing multiple serositis, arthritis and other symptoms, and the incidence rate is as high as 15% to 90% %, the mortality rate can sometimes be as high as 90%. At first Haemophilus parasuis was called Haemophilus parasuis or Haemophilus influenzae swine, but it was later confirmed that factor X (heme and other porphyrins) was not required for its growth, so it was renamed Haemophilus p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/21
Inventor 林标声李晓华林炜明
Owner LONGYAN UNIV