Phenotypic identification method and mass spectrometry method comprehensive microorganism identification system and phenotypic identification method and mass spectrometry method comprehensive microorganism identification method

Abstract

The invention relates to a phenotypic identification method and mass spectrometry method comprehensive microorganism identification system and a phenotypic identification method and mass spectrometry method comprehensive microorganism identification method. The system comprises: a matrix-assisted laser desorption ionization ion source for emitting laser to accelerate ions and lead out, and then carrying out ion focusing; a time-of-flight mass analyzer for making the ions pass through a field-free flight zone and detecting the electrical signals generated under the ion impact effect to form a mass spectrum; a microorganism image acquisition device for acquiring image information of a sample to be detected to obtain phenotypic characteristics; and a computer processing device connected with the devices, wherein the computer processing device comprises a control module, a signal acquisition module and an information processing module, and is used for carrying out machine vision-based pattern recognition according to the acquired image information to obtain the phenotypic identification result of the microorganism and carrying out specific-level and specific-range mass spectrometry identification according to the acquired the mass spectrum and phenotypic identification result combination to obtain the identification result. According to the present invention, the phenotypic identification method and the mass spectrometry method are organically combined so as to obtain the more efficient and accurate identification result compared with the identification result of the traditional method.

Description

technical field [0001] The invention relates to the field of microbial identification, in particular to a microbial identification system and method integrated with phenotypic identification and mass spectrometry. Background technique [0002] Machine Vision (Machine Vision) refers to capturing the target image through the image capture device, transmitting the image to the processor, based on the pixel distribution, brightness, color and other information, to distinguish the parameters such as size, shape and color, and then obtain the judgment result. The application of machine vision in my country originated in the 1980s. It has developed rapidly in recent years and the market has grown significantly. However, at present, machine vision technology is mainly used in industrial fields such as electronics manufacturing, automobiles, pharmaceuticals, and packaging machinery, accounting for 70% of its market share. . Related documents include the patent "A Machine Vision Auto ...

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Relevant documents include the patent "MALDI-TOF-MS method for detecting pathogenic Vibrio", master's degree thesis "MALDIBiotyper new technology for rapid identification and classification of microorganisms", etc., and the combination of machine vision phenotyping identification method and mass spectrometry identification method has not yet been retrieved Applied Related Technologies

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