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Alginate oligomer dairy capable of reducing cholesterol

A technology of fucoidan oligosaccharides and dairy products, applied in dairy products, milk preparations, applications, etc., can solve problems such as difficult to dissolve, large molecular weight, high viscosity, etc., and achieve the effect of inhibiting absorption, inhibiting reaction, and reducing content

Active Publication Date: 2015-12-09
QINGDAO BZ OLIGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alginate is a kind of monomer composed of β-1,4-D-mannuronic acid (M) and α-1,4-L-guluronic acid (G) through 1,4- Linear polysaccharide polymers linked by glycosidic bonds mainly come from brown algae plants such as kelp, sargasso, and macroalgae. They have various physiological activities such as anti-tumor, enhancing immunity, and promoting growth. Substances with high molecular weight, large molecular weight, difficult to dissolve, and high viscosity, have certain limitations in practical application

Method used

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  • Alginate oligomer dairy capable of reducing cholesterol
  • Alginate oligomer dairy capable of reducing cholesterol
  • Alginate oligomer dairy capable of reducing cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of alginate lyase

[0024] (1) To 1000 mL of seawater, add 50 grams of kelp powder and 10 grams of potassium nitrate, adjust the initial pH to 7.0 with NaOH aqueous solution, sterilize and cool to room temperature. (2) Add 100 mL of strain, culture with shaking at 25°C for 48 hours, and centrifuge to get the supernatant. (3), add 10 grams of polyetherimide, and centrifuge to remove the flocculent precipitate. (4) The supernatant is eluted on a Sepharose chromatographic column to obtain alginate lyase.

[0025] 2. Preparation of fucoidan oligosaccharides

[0026] (1) Dissolution of alginate: Dissolve 20 grams of alginate in 2000 mL of distilled water to form a 1% alginate solution, heat and stir at 60° C. on a magnetic stirrer to completely dissolve it. (2) Enzymatic hydrolysis of alginate: add 10 mL of alginate lyase into the dissolved alginate solution, and react on a magnetic stirrer at 60° C. for 12 hours. (3) Inactivation of the enzymolysis soluti...

Embodiment 2

[0043] The specific implementation is as follows:

[0044] 1. Preparation of alginate lyase

[0045] (1) To 1000 mL of seawater, add 50 grams of kelp powder and 10 grams of potassium nitrate, adjust the initial pH to 7.0 with NaOH aqueous solution, sterilize and cool to room temperature. (2) Add 100 mL of strain, culture with shaking at 25°C for 48 hours, and centrifuge to get the supernatant. (3), add 10 grams of polyetherimide, and centrifuge to remove the flocculent precipitate. (4) The supernatant is eluted on a Sepharose chromatographic column to obtain alginate lyase.

[0046] 2. Preparation of fucoidan oligosaccharides

[0047] (1) Dissolution of alginate: Dissolve 20 grams of alginate in 2000 mL of distilled water to form a 1% alginate solution, heat and stir at 60° C. on a magnetic stirrer to completely dissolve it. (2) Enzymatic hydrolysis of alginate: add 10 mL of alginate lyase into the dissolved alginate solution, and react on a magnetic stirrer at 60° C. for ...

Embodiment 3

[0064] The specific implementation is as follows:

[0065] One, the preparation of alginate lyase:

[0066] (1) Seawater 1000mL, add 50 grams of kelp powder and 10 grams of potassium nitrate, adjust the initial pH to 7.0 with NaOH aqueous solution, sterilize and cool to room temperature. (2) Add 100mL strains, culture with shaking at 25°C for 48h, and centrifuge to get the supernatant. (3) Add 10 grams of polyetherimide, and centrifuge to remove the flocculent precipitate. (4) The supernatant is eluted on a Sepharose chromatographic column to obtain alginate lyase.

[0067] 2. Preparation of fucoidan oligosaccharides:

[0068] (1) Dissolution of alginate: Dissolve 20 grams of alginate in 2000 mL of distilled water to form a 1% alginate solution, and heat and stir at 60° C. on a magnetic stirrer to completely dissolve it. (2) Enzymatic hydrolysis of alginate: Add 10 mL of alginate lyase into the dissolved alginate solution, and react on a magnetic stirrer at 60° C. for 12 h...

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Abstract

The invention relates to the field of foods, in particular to an alginate oligomer dairy capable of reducing cholesterol. The dairy disclosed by the invention contains alginate oligomers of which the mass percentage is 1-10%, and the molecular weight scope of the alginate oligomers is 2000-3000Da. The level of serum cholesterol can be obviously reduced by restraining the absorption of cholesterol by small intestines. After the alginate oligomers are prepared into the diary disclosed by the invention, the alginate oligomers are easier to absorb, and the diary has better effects of reducing the content of the cholesterol and the content of triglyceride in blood than a general dairy and initial alginate oligomer powder. Besides, the alginate oligomers interact with orotic acid in the dairy to restrain a reaction of synthesizing the cholesterol by livers and restrain the absorption of cholesterol by small intestines, so that the content of the cholesterol and the content of the triglyceride in the blood can be obviously reduced by the alginate oligomer dairy.

Description

technical field [0001] The invention relates to the field of food, in particular to a cholesterol-lowering fucoidan oligosaccharide dairy product. Background technique [0002] In recent years, with the improvement of people's living standards and the Westernization of eating habits, the public's intake of meat and fried food is increasing. Studies have shown that the prevalence of hypercholesterolemia among people over 20 years old in my country is 9.0%, and the prevalence of borderline hypercholesterolemia is 22.5%. This means that the number of people suffering from hypercholesterolemia in my country is nearly 100 million. The average serum total cholesterol (TC) and triglyceride (TG) levels of Chinese adults increased by 23.9% and 42.7%, respectively. If effective intervention measures are not taken, the prevalence of atherosclerotic cardiovascular disease in my country will increase rapidly in the near future. [0003] Alginate oligosaccharides, also known as alginat...

Claims

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Application Information

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IPC IPC(8): A23C9/13
Inventor 张斌管昶王丽丽王静徐小龙
Owner QINGDAO BZ OLIGO BIOTECH
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