Method for inducing chlorella vulgaris ZF algal strains to efficiently accumulate linolenic acid

A common chlorella, linolenic acid technology, applied in the biological field, can solve the problems of high cost, complicated technical process, increased production cost and difficulty in technology promotion, etc., and achieves the effects of easy cultivation, short cycle and low cost

Inactive Publication Date: 2015-12-09
SHANDONG UNIV OF TECH
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Problems solved by technology

Although the above-mentioned relevant patented technologies related to the purification and preparation of D-linolenic acid are advanced, none of them involve the content of plant growth regulators inducing the accumulation of linolenic acid in Chlorella vulgaris, and the technical process is relatively complicated and the cost is high, which will undoubtedly increase Reduced the difficulty of production cost and technology promotion

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, adopt the following steps:

[0013] 1) Preparation of algae liquid: under the condition of 23°C, light intensity 1800lx and light-dark ratio of 12h / 12h, the cells of Chlorella vulgaris ZF algae strain were cultivated for 28 days to the logarithmic growth phase with 1 / 4 times BG11 medium to obtain For the algae liquid induced by plant growth regulators, add 1-fold concentration of BG11 nutrient salts every 14 days during the culture period;

[0014] 2) Accumulation of linolenic acid: Take 200mL of algae liquid in the logarithmic growth phase and place it in a 300mL triangular conical flask, then add the plant growth regulator 6-BA produced by Beijing Suo Laibao Technology Co., Ltd. to make the plant growth regulator 6-BA The concentration in the algae liquid is 1.00mg / L, and the induction treatment is carried out at 23°C for 14 days. During the treatment stage, artificial shaking algae is not less than 3 times a day, and the time interval between adjacent s...

Embodiment 2

[0015] Embodiment 2, adopt the following steps:

[0016] 1) Preparation of algae liquid: under the conditions of 24°C, light intensity 2000lx and light-dark ratio of 12h / 12h, the cells of Chlorella vulgaris ZF algae strain were cultivated in 1 / 4 times BG11 medium for 28 days to logarithmic growth phase, and obtained For the algae liquid induced by plant growth regulators, add 1-fold concentration of BG11 nutrient salts every 14 days during the culture period;

[0017] 2) Accumulation of linolenic acid: Take 200mL of algae liquid in the logarithmic growth phase and place it in a 300mL triangular conical flask, then add the plant growth regulator 6-BA produced by Beijing Suo Laibao Technology Co., Ltd. to make the plant growth regulator 6-BA The concentration in the algae liquid is 0.95mg / L, and the induction treatment is carried out at 24°C for 15 days. During the treatment stage, the artificial shake algae is not less than 3 times a day, and the time interval between adjacent ...

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Abstract

The invention provides a method for inducing chlorella vulgaris ZF algal strains to efficiently accumulate linolenic acid. The method is characterized by adopting the following steps : 1) preparation of algae solution : under the conditions of the temperature being 23+ / -2 DEG C, the light intensity being 1900+ / -1001 x and the ratio of light to dark being 12h / 12h, a 1 / 4 BG11 culture medium is adopted for culturing chlorella vulgaris ZF algal strain cells from 28 days to an exponential phase, an algae solution used for plant growth regulator induction is obtained, and BG11 nutritive salt with twice concentration is added once every 14 days during the culture period; 2) accumulation of linolenic acid: 200ml of the algae solution in the exponential phase is taken and placed into a 300ml conical flask, then a plant growth regulator 6-BA is added, the concentration of the plant growth regulator 6-BA in the algae solution is 1+ / -0.05 mg / L, induction treatment is performed at 23+ / -2 DEG C for 14+ / -1 days, manual shaking of the algae solution is not less than three times every day in the treatment stage, time intervals between adjacent shaking of the algae solution are not less than 2 hours, and the accumulation of linolenic acid in the algae solution is realized. The method is simple and feasible, the cost is low, and the accumulation of the linolenic acid content can be remarkably improved in a short term.

Description

technical field [0001] The invention provides a method for inducing chlorella vulgaris ZF strains to efficiently accumulate linolenic acid, which belongs to the field of biotechnology. Background technique [0002] Chlorella (Chlorella) is a common aquatic single-celled algae, which is an important genus in the family Chlorellaceae. It has a wide ecological distribution and is distributed in both seawater and freshwater. Currently, it includes about 10 species. Chlorella cells are rich in protein (50% of dry weight), essential amino acids, polysaccharides, lipids (results of fatty acid accumulation), chlorophyll, carotene, and vitamins, etc., used in health food, aquaculture (feed additives) ), cosmetics, medicine and other fields are widely used, and the domestic and foreign markets are in short supply. Compared with the United States, Japan, Israel and other countries, the level of industrialized large-scale cultivation of Chlorella in my country is relatively lagging beh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
Inventor 高政权李国强孟春晓吴冠勋郭艳芸付圣贵沈义成孙海风邓素贞胡硕陈国强张瑞豪
Owner SHANDONG UNIV OF TECH
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