Method for inducing chlorella vulgaris ZF algal strains to efficiently accumulate linolenic acid
A common chlorella, linolenic acid technology, applied in the biological field, can solve the problems of high cost, complicated technical process, increased production cost and difficulty in technology promotion, etc., and achieves the effects of easy cultivation, short cycle and low cost
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Embodiment 1
[0012] Embodiment 1, adopt the following steps:
[0013] 1) Preparation of algae liquid: under the condition of 23°C, light intensity 1800lx and light-dark ratio of 12h / 12h, the cells of Chlorella vulgaris ZF algae strain were cultivated for 28 days to the logarithmic growth phase with 1 / 4 times BG11 medium to obtain For the algae liquid induced by plant growth regulators, add 1-fold concentration of BG11 nutrient salts every 14 days during the culture period;
[0014] 2) Accumulation of linolenic acid: Take 200mL of algae liquid in the logarithmic growth phase and place it in a 300mL triangular conical flask, then add the plant growth regulator 6-BA produced by Beijing Suo Laibao Technology Co., Ltd. to make the plant growth regulator 6-BA The concentration in the algae liquid is 1.00mg / L, and the induction treatment is carried out at 23°C for 14 days. During the treatment stage, artificial shaking algae is not less than 3 times a day, and the time interval between adjacent s...
Embodiment 2
[0015] Embodiment 2, adopt the following steps:
[0016] 1) Preparation of algae liquid: under the conditions of 24°C, light intensity 2000lx and light-dark ratio of 12h / 12h, the cells of Chlorella vulgaris ZF algae strain were cultivated in 1 / 4 times BG11 medium for 28 days to logarithmic growth phase, and obtained For the algae liquid induced by plant growth regulators, add 1-fold concentration of BG11 nutrient salts every 14 days during the culture period;
[0017] 2) Accumulation of linolenic acid: Take 200mL of algae liquid in the logarithmic growth phase and place it in a 300mL triangular conical flask, then add the plant growth regulator 6-BA produced by Beijing Suo Laibao Technology Co., Ltd. to make the plant growth regulator 6-BA The concentration in the algae liquid is 0.95mg / L, and the induction treatment is carried out at 24°C for 15 days. During the treatment stage, the artificial shake algae is not less than 3 times a day, and the time interval between adjacent ...
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