Method of promoting hydrolysis of juglan regia ketone glucoside in immature juglans mandshurica skin into juglan regia ketone, and content measurement method of juglone and juglan regia ketone
A technology of juglone glycosides and juglone, which is applied in the field of content determination of juglone and juglone, can solve the problems of large differences in juglone and difficulty in controlling the quality of anticancer drugs, and achieve high accuracy, accurate content, and quality assurance and stability effects
Inactive Publication Date: 2015-12-09
HEILONGJIANG ACAD OF TCM
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Problems solved by technology
[0006] The purpose of the present invention is to solve the problem that the quality of anticancer drugs prepared by using Beiqinglongyi is difficult to control due to the large difference of juglone quinone in Beiqinglongyi due to different producing areas, and to provide a method to promote juglone glycosides in Beiqinglongyi Method for hydrolyzing juglone and content determination method of juglone and juglone
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specific Embodiment approach 1
[0015] Specific implementation mode one: the method for promoting the hydrolysis of juglone glycosides in Beiqinglongyi to juglone according to the following steps:
[0016] Add Qinglongyi powder into methanol solution for ultrasonic treatment, naturally cool to room temperature and then filter, take the filtrate and add acidic solution to reflux for hydrolysis, that is, to realize the hydrolysis of bound juglone glycosides in Beiqinglongyi to free juglone.
[0017] Among them, the ratio of Qinglongyi powder to methanol solution is 0.5g-3g:25ml; the ultrasonic treatment time is 40min, and the ultra-processing frequency is 30-70kHZ; the reflux temperature is 70±5℃, and the reflux time is 1±0.2h.
specific Embodiment approach 2
[0018] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the concentration of the methanol solution is 20% to 100%. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0019] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the volume ratio of the filtrate to the acidic solution is 15:1.5-14. Other steps and parameters are the same as those in Embodiment 1 or 2.
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Abstract
A method of promoting hydrolysis of juglan regia ketone glucoside in immature juglans mandshurica skin into juglan regia ketone, and a content measurement method of juglone and juglan regia ketone. The invention relates to a method of hydrolysis of juglan regia ketone glucoside into juglan regia ketone and a content measurement method of the juglone and the juglan regia ketone, and solves the problem that the quality of anti-cancer drugs prepared from the immature juglans mandshurica skin is difficult to control due to huge difference in the juglone in the immature juglans mandshurica skin from different regions. The hydrolysis method includes following steps: 1) adding an immature juglans mandshurica skin powder into a methanol solution and performing ultrasound treatment; 2) cooling and filtering the liquid to obtain a filtrate; and 3) adding an acidic solution to the filtrate and performing reflux hydrolysis to hydrolyze the conjugated juglan regia ketone glucoside in the immature juglans mandshurica skin into free-type juglan regia ketone. The metering method is carried out through a high-performance liquid gradient detection method. The method in the invention promotes the conjugated juglan regia ketone glucoside in the immature juglans mandshurica skin to be hydrolyzed to form the free-type juglan regia ketone, so that the contents of main anti-tumor active components in the immature juglans mandshurica skin from different regions are stable, thereby ensuring the quality and stability of the anti-cancer drugs from the immature juglans mandshurica skin.
Description
technical field [0001] The invention relates to a method for hydrolyzing juglone glycosides into juglone and a content determination method for juglone and juglone. Background technique [0002] Cancer is a serious disease that threatens human life. The research and development of anticancer drugs has always been a hot spot in the world's medical circles. Cancer recurrence and metastasis are the two main causes of death in cancer patients, marking the failure of treatment. Traditional Chinese medicine plays an increasingly important role in the field of cancer treatment through multi-channel comprehensive treatment and mobilization of the body's overall defense system. [0003] Beiqinglongyi, also known as walnut green skin, is the immature exocarp of Juglans mandshurica Maxim. It is mainly distributed in the eastern part of Heilongjiang Province in my country, in mountainous areas such as Xiaoxing'an Mountains, Wanda Mountains, Laoye Mountains and Zhangguangcai Mountains....
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IPC IPC(8): G01N30/02C07C50/32C07C46/00
Inventor 王伟明初东君霍金海张海燕任晓蕾曹贵阳
Owner HEILONGJIANG ACAD OF TCM