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A method for efficiently enriching diarylheptanes in Saxifrage tangutata

A technology of diaryl heptane and saxifrage tangutica, which is applied in the field of high-efficiency enrichment of diaryl heptane compounds in saxifrage tangutica, and can solve problems that have not involved the basis of effective substances in saxifrage tangutica , to achieve the effects of easy large-scale implementation, environmental friendliness, stable and controllable quality

Inactive Publication Date: 2017-11-28
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research reports on Saxifrage tangutatus are limited to the isolation of 4 flavonoids and 2 sterols (Yang Aimei, Li Yulan. Research on the chemical constituents of Tibetan medicine Ganqing saxifrage, Chinese Journal of Traditional Chinese Medicine, 2011, 26 (9): 1975-1977.), did not involve the material basis of the efficacy of Tangutia saxifrage in treating hepatitis as recorded in "Cihai of Traditional Chinese Medicine"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 The method for efficiently enriching diarylheptane compounds in Saxifrage tangutata comprises the following steps:

[0020] (1) Extraction: After crushing 2.5 Kg of Saxifrage tangutica medicinal materials to 50 meshes, adding an ethanol solution with a volume fraction of 40% for alcohol extraction, and obtaining the extract of Saxifrage tanguticae by filtration.

[0021] Among them: the alcohol extraction conditions refer to the solid-liquid ratio of 1kg:6L, the temperature of 85°C, the extraction times of 3 times, and each extraction time of 1 h.

[0022] (2) Alcohol-resistant membrane for impurity removal: After treating the saxifrage extract with a molecular weight of 8000 with an alcohol-resistant membrane, the permeate was collected, and the permeate was subjected to vacuum at 0.06 MPa and 65 °C Dry under reduced pressure to obtain 58.4 g crude sample of diarylheptane compounds from Saxifrage tangutata.

[0023] (3) Reverse-phase solid-phase extraction c...

Embodiment 2

[0026] Example 2 The method for efficiently enriching diarylheptane compounds in Saxifrage tangutata comprises the following steps:

[0027] (1) Extraction: After crushing 5.5 Kg of Saxifrage tangutica medicinal materials to 120 meshes, adding an ethanol solution with a volume fraction of 95% for alcohol extraction, and obtaining the extract of Saxifrage tanguticae by filtration.

[0028] Among them: the alcohol extraction conditions refer to the solid-liquid ratio of 1kg:10L, the temperature of 65°C, the number of extractions once, and each extraction time of 3 h.

[0029] (2) Alcohol-resistant membrane for impurity removal: After treating the saxifrage extract with a molecular weight of 12,000 with an alcohol-resistant membrane, the permeate was collected, and the permeate was subjected to vacuum at a degree of 0.09 MPa and a temperature of 55°C. Dry under reduced pressure to obtain 124.8 g crude sample of diaryl heptane compounds from Saxifrage tangutata.

[0030] (3) Reve...

Embodiment 3

[0033] Example 3 The method for efficiently enriching diarylheptane compounds in Saxifrage tangutica comprises the following steps:

[0034] (1) Extraction: After crushing 2.0 Kg of Saxifraga tangutica medicinal material to 80 meshes, adding an ethanol solution with a volume fraction of 75% for alcohol extraction, and obtaining the extract of Saxifraga tanguticae by filtration.

[0035] Among them: the alcohol extraction conditions refer to the solid-liquid ratio of 1kg:8L, the temperature of 75°C, the extraction times of 2 times, and each extraction time of 2h.

[0036] (2) Alcohol-resistant membrane for impurity removal: After treating the saxifrage extract with a molecular weight of 10,000 through an alcohol-resistant membrane, the permeate was collected, and the permeate was subjected to vacuum at a vacuum of 0.08 MPa and a temperature of 60°C. Dry under reduced pressure to obtain 43.9 g crude sample of diaryl heptane compound from Saxifrage tangutata.

[0037] (3) Revers...

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PUM

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Abstract

The present invention relates to a method for efficiently enriching diaryl heptane compounds in Saxifrage tangutica, the method comprising the following steps: (1) Extraction: crushing the medicinal material of Saxifraga tangutica and carrying out alcohol extraction, and obtaining Tanggu S. saxifrage extract; (2) alcohol-resistant film for impurity removal: the extract of saxifrage tangutica is treated with an alcohol-resistant film, and the permeate is collected, and the permeate is dried under reduced pressure to obtain saxfrage tangutata Grass diaryl heptane compound crude sample; (3) Reverse-phase solid phase extraction column enrichment: add water to dissolve in the described saxifrage diaryl heptane compound crude sample, then pass through the reverse phase solid phase extraction column Separation, and carry out step gradient elution, collect 50% ~ 70% methanol eluate; (4) described 50% ~ 70% methanol eluate is dried under reduced pressure to obtain Saxifrage tangutata diaryl heptane compound components. The invention has simple process, easy large-scale implementation and stable and controllable quality.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a method for efficiently enriching diaryl heptane compounds in Saxifrage tangutata. Background technique [0002] Tangut saxifrage ( Saxifraga tangutica Engl.), also known as Ganqing saxifrage, is a member of the saxifrage family ( Saxifragaceae ) Saxifraga ( Saxifraga ) annual evergreen herb, Tibetan medicine name: "Songjidi", traditional Chinese medicine called "Dieda", mainly distributed in Qinghai, Gansu, Tibet, Sichuan, and Bhutan and Kashmir at an altitude of 2900~4900 meters Under coniferous thickets. Tangut saxifrage is a commonly used Tibetan medicine, and the whole herb can be used as medicine. "Chinese Medicine Cihai" records: its nature and taste are slightly bitter, pungent, and cold. Main effects: clearing the liver and gallbladder, invigorating the spleen and strengthening the stomach. Indications: hepatitis, cholecystitis, infl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C07H1/08C07C45/79C07C49/245A61K31/7032A61K31/12A61K36/185
Inventor 党军张莉邵赟陶燕铎王启兰梅丽娟苑祥
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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