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Marine microorganism strain Y112 and alpha-cyclodextrin glycosyltransferase produced by strain

A marine microorganism, glucose-based technology, applied in the field of α-cyclodextrin glucosyltransferase

Active Publication Date: 2015-12-16
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art enzymatic method to produce a mixture of various cyclodextrins. After long-term development and research by the inventors on marine microorganisms and their enzyme products and production practices, they develop and provide a single α-cyclodextrin glucose Marine microbial strain Y112 and its α-cyclodextrin glucosyltransferase

Method used

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  • Marine microorganism strain Y112 and alpha-cyclodextrin glycosyltransferase produced by strain
  • Marine microorganism strain Y112 and alpha-cyclodextrin glycosyltransferase produced by strain
  • Marine microorganism strain Y112 and alpha-cyclodextrin glycosyltransferase produced by strain

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Embodiment 1

[0065] Embodiment 1—breeding and identification of marine microbial strain Y112 producing α-CGTase:

[0066] 1. Preparation of crude enzyme solution

[0067] After inoculating 30 mL of α-CGTase-producing marine microbial strain Y112 in 250 mL of Erlenmeyer bottle-packed seed culture medium, activate it for 22 hours at 30°C and 200 r / min, and inoculate with 4% (V / V) inoculum until 30 mL of fermentation medium is installed. Incubate in a 250mL Erlenmeyer flask at 30°C and 200r / min for 60h, collect the fermentation broth and centrifuge for 15min under the condition of 10000g centrifugal force, and the supernatant is the crude enzyme solution. The composition of the seed medium and the composition of the fermentation medium are the same as above.

[0068] 2. Breeding of α-cyclodextrin glucosyltransferase-producing bacteria

[0069] 1), Screening of strains producing CGTase:

[0070]Add 0.03% (w / v) phenolphthalein and 0.01% (w / v) methyl orange on the alkaline agar medium as an a...

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Abstract

The invention relates to a marine microorganism strain Y112 for producing alpha-cyclodextrin glycosyltransferase and enzyme produced by the strain. Upon 16SrDNA sequence analysis and in coordination with physiological and biochemical characteristics, the strain belongs to bacillus; and through sequencing, 16SrDNA sequence is about 1420bp long, and base composition is as shown in Figure 1. The marine microorganism strain is extensively applicable to medicine, food, chemical industry, cosmetics, industry, agriculture, analytical chemistry and like aspects.

Description

technical field [0001] The invention relates to the field of marine microorganisms, in particular to a marine microorganism strain Y112 and the alpha-cyclodextrin glucosyltransferase produced therefrom. Background technique [0002] Cyclodextrin (cyclodextrin, abbreviated as CD) is formed by α-1,4-glucosidic linkages, cyclic maltooligosaccharides without reducing ends. Cyclodextrins usually consist of 6, 7 or 8 glucose residues and are called α-, β-, or γ-cyclodextrins, respectively. In addition to these three commonly used cyclodextrins, there are also δ-, ε-, ζ-, and η-cyclodextrins (composed of 9 to 12 glucose units, respectively). Cyclodextrins have the ability to encapsulate all or part of a variety of chemicals into cavities, thereby altering the physical and chemical properties of these compounds. Cyclodextrin is converted from starch by cyclodextrin glucosyltransferase (CGTase), and the main product is a mixture of α-, β-, and γ-cyclodextrin. Because the cyclodext...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/10C12R1/07
Inventor 孙谧郝建华
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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