Primer pair for detecting caprine parainfluenza virus type 3 (CPIV3) and peste des petits ruminants virus (PPRV)

A technology of Peste des petits ruminants and primer pairs, applied in the field of animal virology and zoonotics

Inactive Publication Date: 2015-12-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is only RT-PCR method for detecting PPRV clinically, but the RT-PCR method for detecting CPIV3 alone and simultaneously detecting CPIV3 and PPRV has not been put into use yet.

Method used

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  • Primer pair for detecting caprine parainfluenza virus type 3 (CPIV3) and peste des petits ruminants virus (PPRV)
  • Primer pair for detecting caprine parainfluenza virus type 3 (CPIV3) and peste des petits ruminants virus (PPRV)
  • Primer pair for detecting caprine parainfluenza virus type 3 (CPIV3) and peste des petits ruminants virus (PPRV)

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Embodiment Construction

[0036] 1 Primer design and synthesis

[0037] CPIV3 is a newly discovered virus in the laboratory. Although it has been publicly published before, it has not been publicly released. PPRV has been very harmful since it was introduced to most provinces in China in 2014. Both are the main pathogens that cause goat respiratory disease. There are mixed infections, and molecular biology methods are needed to distinguish the two. At present, there is only a RT-PCR method for detecting PPRV in clinical practice. However, the RT-PCR method for detecting CPIV3 alone and detecting both CPIV3 and PPRV has not been put into application.

[0038] Primer design is the key to determining the success or failure of multiplex PCR. The present invention through long-term research and systematic research on the newly discovered virus CPIV3 in our laboratory, through sequence analysis and comparison, respectively selects the conserved regions of CPIV3M protein gene and PPRVN protein gene for primer des...

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Abstract

The invention relates to a primer pair for detecting caprine parainfluenza virus type 3 (CPIV3) and peste des petits ruminants virus (PPRV), and belongs to the field of biotechnology. The invention provides a special primer for detecting viruses, wherein the special primer comprises a primer pair A and a primer pair B, the primer pair A comprises a primer 1 and a primer 2, and the primer pair B comprises a primer 3 and a primer 4; the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are respectively a sequence 1, a sequence 2, a sequence 3 and a sequence 4 in a sequence table. The primer special for CPIV3 and PPRV duplicate RT-PCR detection, provided by the invention is high in sensitivity, can detect the content of RNA as low as about multiplying 1 by 10<-3>ng / micro-liter, and the sensitivity of which is much higher than 1ng / micro-liter. The duplicate RT-PCR detection method provided by the invention is good in specificity, simple and quick to operate, low in requirement on the quality of a detecting material, and convenient to use clinically.

Description

Technical field [0001] The invention belongs to the technical field of animal virology and animal infectious diseases. Specifically, it relates to a primer pair for detecting goat parainfluenza virus type 3 and Peste des petits ruminants virus. Background technique [0002] Goat parainfluenza virus type 3 (Caprineparainfluenzavirustype 3, CPIV3) is a member of the Paramyxoviridae (Paramyxoviridae) paramyxovirinae respiratory virus genus (Respirovirus), which can cause respiratory infections of varying severity in goats, such as colds. , Cough, increased ocular and nasal secretions, bronchitis, bronchiolitis and pneumonia. In 2014, our laboratory was the first to isolate and identify the virus from goat clinical disease materials that showed severe respiratory symptoms (the first time at home and abroad), and the artificial infection test proved that the virus is pathogenic to goats. Pestedespetitsruminantsvirus (PPRV) is a member of the Paramyxovirinae measles virus genus (Morb...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/701C12Q2600/16
Inventor 郝飞李文良毛立杨蕾蕾江杰元张纹纹王钟毓
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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