Application of hexa-tert-butyl-hexahexyloxy-hexabenzo[ab, de, lm, op, rs, uv] corone as maldi-tof MS matrix in detection of small molecules

Abstract

The invention discloses an application of 6-tertiary butyl-6-hexyloxy-6-benzo[a,b,de,lm,op,rs,uv]coronene as an MALDI-TOF MS matrix in micromolecule detection. Small molecules comprise amino acid, medicine micromolecules, saccharide micromolecules, aromatic hydrocarbon compounds and the like. 6-tertiary butyl-6-hexyloxy-6-benzo[a,b,de,lm,op,rs,uv]coronene based on nano graphene has the characteristic of graphene, can be dissolved into an organic solvent, is not prone to fly away from a target plate as the MALDI-TOF MS matrix, and rarely has pollution to an ion source, the defect that a traditional matrix is poor in micromolecule measuring effect is overcome, and 6-tertiary butyl-6-hexyloxy-6-benzo[a,b,de,lm,op,rs,uv]coronene has the good effect when used for detecting MALDI-TOFMS of multiple kinds of micromolecules.

Description

technical field [0001] The invention belongs to the application technical field of MALDI-TOFMS, in particular to a kind of hexa-tert-butyl-hexahexyloxy-hexabenzo[ab, de, lm, op, rs, uv] corona (HBCCs) as MALDI-TOFMS The application of the matrix in the detection of small molecules (molecular weight less than 500Da). Background technique [0002] In the late 1980s, a new type of soft ionization biological mass spectrometry, matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOFMS), was developed, which has the characteristics of good accuracy, high sensitivity and high resolution. MALDI-TOFMS is a simple and fast technique mainly used to analyze large biomolecules, so it has been widely used to analyze proteins, peptides, nucleotides, etc. It has better salt tolerance, lower requirements for sample pretreatment, and greatly saves labor. [0003] In MALDI-TOFMS, the matrix plays the role of absorbing and transmitting laser energy and ionizing...

AI Technical Summary

Problems solved by technology

However, traditional substrates such as α-cyano-4-hydroxycinnamic acid (CHCA), dihydroxybenzoic acid (DHB) and 2,4,6-trihydroxyacetophenone (THAP) are easily cleaved under laser radiation, so In the low molecular weight region (<500Da), there will be undesirable matrix ion interference, and at the same time, due to the heterogeneous co-crystallization of the matrix and the analyte, the detector will form saturation hotspots, which will cause difficulties in the observation and characterization of small molecules, so that it is difficult Analyze Small Molecules Using Traditional Matrices

In recent years, studies have found that new carbon materials such as graphene, graphene oxide, nitrogen-doped graphene, carbon dots, and carbon nanotubes can be used as MALDI substrates for the measurement of small molecules. The target plate is not firmly bonded, and it is easy to fly away from the target plate to contaminate the ion source during the ionization process. At the same time, materials such as graphene and carbon nanotubes are not well dispersed in the solution, and they are easy to agglomerate, which has the problem of unsatisfactory reproducibility.

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