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Method and device for plant seed mutagenesis
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A technology for plant seeds and seeds, applied in the field of plant seed mutagenesis and plant seed mutagenesis devices, can solve problems such as uneven action and cleaning of chemicals, failure of mutagenesis, instability of mutation, etc.
Active Publication Date: 2017-09-15
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology
[0002] The seed mutagenesis before the present invention is all done manually, and there are the following technical problems: 1) The seed mutagen is a strong carcinogenic agent, which is harmful to people; cause mutagenesis failure; 3) the treatment time is not accurate, the mutation rate is often unstable, and it is impossible to analyze, improve and improve the mutagenesis technique; 4) a large number of mutagenic plant seeds often have uneven chemical action and cleaning, and mutation Unstable, unable to perform molecular biologyscreening analysis of mutants, leading to failure of seed breeding projects
Method used
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[0045] Effects: Treat 500 grams of seeds, select 20,000 seeds with a germination rate of about 95%, 17,380 seedlings, 10,759 plants, and 5,647 seeds, with a mutation rate of 1-3 mutation sites / kb.
Embodiment 2
[0047] Treat Yunyan 87
[0048] 1) Use 50% bleaching water to treat for 6 minutes, then centrifuge and filter to dry;
[0049] 2) After adding the rinse agent, 1 minute, centrifuge and dry to remove the bleaching water component;
[0050] 3) At room temperature, soak tobacco seeds in deionized water for 10-12 hours, then centrifuge and dry;
[0052] 5) After adding the rinsing agent, rotate the drum for 1 minute, centrifugally filter and dry, and repeat rinsing 8 times in total.
[0053] The effect produced: 200 grams of seeds were treated, 20,000 seeds were selected, the germination rate was about 75%, 13,090 seedlings were planted, 10,812 plants were planted, 2,347 seeds were obtained, and the mutation rate was 10-13 mutation sites / kb.
Embodiment 3
[0055] Process K326
[0056] 1) Treat with 10% bleaching water for 12 minutes, then centrifuge and filter to dry;
[0057] 2) After adding the rinse agent, 1 minute, centrifuge and dry to remove the bleaching water component;
[0058] 3) At room temperature, soak tobacco seeds in deionized water for 10-12 hours, then centrifuge and dry;
[0060] 5) After adding the rinsing agent, rotate the drum for 1 minute, centrifugally filter and dry, and repeat rinsing 8 times in total.
[0061] Effects: Treat 50 grams of seeds, select 20,000 seeds with a germination rate of about 55%, 9,700 seedlings, 7,965 plants, and 1,765 seeds, with a mutation rate of 20-25 mutation sites / kb.
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Abstract
The invention discloses a plant seed mutagenesis method and device. The method provided by the invention includes the steps of: rinsing; cleaning; soaking in deionized water; mutagen treatment; and mutagen removal. The device provided by the invention includes: a box, a roller and a driving motor. The box is provided with the roller, the roller is connected to the driving motor, the roller is in connection with a water supplypipe and a water outlet pipe, the water supplypipe is provided with a treatment agent pipe that is communicated with a treatment agent box, and the treatment agent pipe is equipped with a feeding gate valve, which is connected to an automatic controller. According to the invention, the driving motor drives the roller, the roller rotates to stir a seed treatment liquid so as to conduct mutagenesis on seeds. The water supply pipe communicated with the roller is connected to the treatment agent pipe, the treatment agent pipe is provided with the feeding gate valve, and the automatic controller controls the feeding gate valve, the roller and other components so as to adapt to each step of seed mutagenesis and realize full automation of the seed mutagenesis treatment process. Without the need of long-term artificial operation, the method and the device reduce the labor intensity, and guarantee that the technical staff can avoid mutagen damage.
Description
technical field [0001] The invention belongs to the technical field of agricultural seed breeding, and in particular relates to a plant seed mutagenesis method and a plant seed mutagenesis device with simple structure, convenient operation and easy observation. Background technique [0002] The seed mutagenesis before the present invention is all done manually, and there are the following technical problems: 1) The seed mutagen is a strong carcinogenic agent, which is harmful to people; cause mutagenesis failure; 3) the treatment time is not accurate, the mutation rate is often unstable, and it is impossible to analyze, improve and improve the mutagenesis technique; 4) a large number of mutagenic plant seeds often have uneven chemical action and cleaning, and mutation Unstable, unable to perform molecular biologyscreening analysis on mutants, leading to failure of seed breeding projects. Therefore, it is of great practical significance to develop a mutagenesis method for p...
Claims
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Application Information
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