Fast propagation method of Bletilla Striata(Thunb.)Reichb.f.
A Bletilla striata and fast technology is applied in the field of rapid propagation of Bletilla striata, can solve the problems of improper raw material ratio, high cost of Bletilla striata seedlings, increase production cost and the like, and achieve the effects of improving quality, reducing cultivation cost and increasing yield
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Embodiment 1
[0021] A method for rapidly multiplying bletilla striata, comprising the following steps:
[0022] (1) Select the bletilla striata fruit that is ripe and plump in the same year without mildew and rot, soak it in saturated washing powder water for 8 minutes, then rinse it under the tap water pipe for 3 hours, and then wash it under sterile conditions with volume concentration Soak it in 75% alcohol for 4 minutes, then turn it into a sodium hypochlorite solution with a volume concentration of 10% and soak it for 14 minutes, then rinse it with sterile water for 5 times, then cut the fruit, take out the seeds, and spread the seeds evenly inoculated on the sprout medium;
[0023] (2) Place the medium inoculated in step 1) in an environment where the temperature is 24° C., the light time is 11 h / day, and the light intensity is 1990 LX, and cultivated for one month to obtain new shoots;
[0024] (3) transfer the sprouts obtained by culturing in step 2) to place in the strong seedlin...
Embodiment 2
[0028] A method for rapidly multiplying bletilla striata, comprising the following steps:
[0029] (1) Select the bletilla striata fruit that is ripe and plump in the same year without mildew and rot, soak it in saturated washing powder water for 12 minutes, then rinse it under the tap water pipe for 5 hours, and then wash it under sterile conditions with volume concentration Soak it in 75% alcohol for 6 minutes, then transfer it to a sodium hypochlorite solution with a volume concentration of 10% and soak it for 16 minutes, then rinse it repeatedly with sterile water for 6 times, then cut the fruit, take out the seeds, and spread the seeds evenly inoculated on the sprout medium;
[0030] (2) Place the medium inoculated in step 1) in an environment with a temperature of 26°C, an illumination time of 13h / day, and an illumination intensity of 2100LX for one month to obtain new shoots;
[0031] (3) It is 26 ℃ to be placed in the strong seedling culture medium that the sprout tra...
Embodiment 3
[0038] A method for rapidly multiplying bletilla striata, comprising the following steps:
[0039] (1) Select the bletilla striata fruit that is ripe and plump in the same year without mildew and rot, soak it in saturated washing powder water for 10 minutes, then rinse it under the tap water pipe for 4 hours, and then wash it under sterile conditions with volume concentration Soak it in 75% alcohol for 5 minutes, then transfer it to a sodium hypochlorite solution with a volume concentration of 10% and soak it for 15 minutes, then rinse it repeatedly with sterile water for 5 times, then cut the fruit, take out the seeds, and spread the seeds evenly inoculated on the sprout medium;
[0040] (2) Place the medium inoculated in step 1) in an environment where the temperature is 25° C., the light time is 12 h / day, and the light intensity is 2000 LX, and cultivated for one month to obtain new shoots;
[0041](3) It is 25 ℃ to be placed in the strong seedling culture medium that the ...
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