A method for killing and tracking bacteria by coating bacteria with self-assembled gold nanoshells and generating photothermolysis and luminescence with laser
A gold nanoshell, self-assembly technology, applied in the direction of diagnosis, heating, radiation therapy, etc. using light, which can solve the problem of lack of in-depth understanding of the characteristics of microbial adsorption and reduction.
Active Publication Date: 2017-06-23
郭文硕
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Problems solved by technology
The interaction between bacteria and metal ions and the role of organisms in it have been studied by scientists, but about gold ions (Au 3+) The characteristics of microbial adsorption and reduction and their applications are not well understood
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[0130] Regarding the technology, means and effects used in the present invention, preferred embodiments are listed below and described in detail with the accompanying drawings. It is believed that the above-mentioned purpose, structure and characteristics of the present invention can be obtained from a deep and specific understanding.
[0131] The method designed by the present invention utilizes nanomaterials coated with bacteria to cover gold nanoshells with the help of laser to generate photothermolysis and luminescence to kill and track bacteria. Here, the present invention selects Escherichia coli BL21 strain, Bacillus subtilis and drug-resistant Staphylococcus aureus as biological template. The present invention uses the solution containing gold ions to mix and cultivate these three kinds of bacteria respectively, because there is an S layer composed of glycoproteins (glycoproteins) on the surface of the bacteria, and the carboxyl group of the glycoproteins on the S layer...
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Abstract
The invention provides a method of coating bacteria with a self-assembled gold nano shell and killing and tracking bacteria by means of photothermolysis and luminescence generated by laser, which can kill Gram-positive, negative and drug-resistant bacteria. The present invention can self-assemble a layer of gold nanoshell on the surface of bacteria without adding any reducing agent. Since gold has very good light-to-heat conversion efficiency, the bacterial gold can be irradiated by linear continuous and nonlinear femtosecond lasers. Nanomaterials generate heat and kill bacteria efficiently, that is, photothermolysis effect; and when irradiated with nonlinear femtosecond laser, the bacterial gold nanomaterials not only generate heat, but also have the optical properties of luminescence, killing bacteria The more dead bacteria, the stronger the luminescence produced, and the continuous light emission will not cause photobleaching; with the help of this luminescence, it can be used as an indicator to track and confirm the survival, movement and location of bacteria.
Description
technical field [0001] The present invention relates to a very simple and efficient way to kill, and in particular to a method of killing and tracking bacteria using self-assembled gold nanoshell-coated nanomaterials that use laser light to generate photothermolysis and luminescence , can be applied to the killing and tracking of drug-resistant bacteria. Background technique [0002] 1. Traditional sterilization method [0003] 1.1 Antibiotics [0004] Antibiotics are a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants in the course of life, which have anti-pathogen or other activities, and are chemical substances that can interfere with the development and function of other living cells . Antibiotics commonly used in clinical practice include extracts from microbial culture fluids and chemically synthesized or semi-synthesized compounds. There are no less than 10,000 known natural antibi...
Claims
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IPC IPC(8): A61N5/067
CPCA61N5/0624A61B5/0059A61N5/067A61L2/04A61N5/062
Inventor 郭文硕
Owner 郭文硕