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Tissue culture fast propagation method for dendrobium candidum

A technology of tissue culture and rapid propagation of Dendrobium candidum, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of less research on the accumulation of medicinal active ingredients, weak research on field transplanting technology, and the seedling cultivation cycle. Long-term problems, to achieve the effect of reducing the risk of tissue culture, easy to promote and use, and low production cost

Inactive Publication Date: 2016-01-13
DAXIN PRODIVITY PROMOTING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems existing in the current research are proposed: the seedling cultivation period is long and the cost is high; the research on field transplanting technology is relatively weak, and the survival rate of the transplanted seedlings is low; the research on the accumulation law of medicinal active ingredients in the seedling cultivation process less

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of tissue culture rapid propagation method of dendrobium candidum of the present invention is characterized in that: it comprises the following steps:

[0026] 1. The establishment of explants: select 3 years old, strong growth, thick stems, excellent plants without damage by diseases and insect pests, cut off from the ground 8-12cm, get the upper part of the stem, then cut the stem into 3cm in length, with The small stem segments of 1-2 axillary buds are used as explant materials; in order to reduce the source of material pollution, when harvesting stem segments, it should be carried out after more than three consecutive days of fine weather; after removing most of the leaves from the harvested stem segments, wash them with tap water. Rinse for 10 minutes, soak in 0.5% laundry detergent solution for 20 minutes, and then rinse with tap water;

[0027] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with ster...

Embodiment 2

[0034] A kind of tissue culture rapid propagation method of dendrobium candidum of the present invention is characterized in that: it comprises the following steps:

[0035] 1. The establishment of explants: select 3 years old, strong growth, thick stems, excellent plants without damage by diseases and insect pests, cut off from the ground 8-12cm, get the upper part of the stem, and then cut the stem into 4cm in length, with The small stem segments of 1-2 axillary buds are used as explant materials; in order to reduce the source of material pollution, when harvesting stem segments, it should be carried out after more than three consecutive days of fine weather; after removing most of the leaves from the harvested stem segments, wash them with tap water. Rinse for 10 minutes, soak in 0.5% laundry detergent solution for 20 minutes, and then rinse with tap water;

[0036] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with ...

Embodiment 3

[0043] A kind of tissue culture rapid propagation method of dendrobium candidum of the present invention is characterized in that: it comprises the following steps:

[0044] 1. The establishment of explants: select 3 years old, strong growth, thick stems, excellent plants without damage by diseases and insect pests, cut off from the ground 8-12cm, get the upper part of the stem, then cut the stem into 5cm in length, with The small stem segments of 1-2 axillary buds are used as explant materials; in order to reduce the source of material pollution, when harvesting stem segments, it should be carried out after more than three consecutive days of fine weather; after removing most of the leaves from the harvested stem segments, wash them with tap water. Rinse for 10 minutes, soak in 0.5% laundry detergent solution for 20 minutes, and then rinse with tap water;

[0045] ② Disinfection of explants: step ① Soak the obtained explants in 75% alcohol for 30 seconds, rinse them with ster...

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Abstract

The invention discloses a tissue culture fast propagation method for dendrobium candidum. The method is realized by the steps of: explant establishment; explant treatment; primary culture; secondary culture; rooting culture; hardening-seedling; transplanting and the like. Components of a special culture medium are selected for primary culture, secondary culture and rooting culture. The transplanted dendrobium candidum is fast to grow and high in survival rate by virtue of a reasonable hardening-seedling measure, so that purposes of a high propagation coefficient and a good propagation effect are achieved. Moreover, the method disclosed by the invention is low in production cost, and inheritable characters of the obtained tissue culture seedlings are consistent. The method is an effective path for tissue culture fast propagation of dendrobium candidum and is wide in application range.

Description

technical field [0001] The invention relates to plant planting, in particular to a method for tissue culture and rapid propagation of dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of Orchidaceae. Born on the semi-shady rocks in the mountains at an altitude of 1600 meters, it likes warm and humid climates and semi-shady and semi-yang environments, and is not cold-resistant. Generally, they can withstand low temperatures of -5°C. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum is the best product of Dendrobium. It is named for its iron-green skin. Dendrobium candidum has unique medicinal value, and its stem is used as medicine. The name of the traditional Chinese medicine: Dendrobium, belongs to the yin-tonifying medicine in the tonic medicine. "Chinese Pharmacopoeia": Yiwei Shengjin, nourishing yin and clearing heat. The tender stems of a few varieti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄忠勤
Owner DAXIN PRODIVITY PROMOTING CENT
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