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Application of docosapentaenoic acid ethanolamine in improving plant resistance to Botrytis cinerea

A technology of docosapentaenoic acid ethanolamine and plant ash, which is applied in the directions of application, plant growth regulator, plant growth regulator, etc., can solve the problems of less research and application, and can extend the validity period, inhibit growth and spread of diseases , the effect of increasing resistance

Active Publication Date: 2017-05-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are relatively few studies and applications on inducing resistance substances for botrytis cinerea fungal diseases.

Method used

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  • Application of docosapentaenoic acid ethanolamine in improving plant resistance to Botrytis cinerea
  • Application of docosapentaenoic acid ethanolamine in improving plant resistance to Botrytis cinerea
  • Application of docosapentaenoic acid ethanolamine in improving plant resistance to Botrytis cinerea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Measure 103.7mL of ethanol solution of eicosapentaenoic acid ethanolamine (EPEA) at 5mg / mL (the medicine is purchased from SIGMA company); then slowly add 190.3mL of water, and mix the mixed solution evenly, and finally add 6mL of organosilicon solution was stirred evenly to obtain the preparation.

[0042] Get 100 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is three leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control. Randomly take tomato leaf samples sprayed with formulations and controls, use Trizol and SuperscriptII kits produced by Invitrogen, USA to extract, purify, and reverse-transcribe cDNA, and then use the Step ONE Plus Real-TimePCR System produced by Applied Biosystems, USA The instrument and the company's SYBR RT-PCR Kit fluorescent dye ki...

Embodiment 2

[0046] Measure 165.9 mL of ethanol solution of eicosapentaenoic acid ethanolamine (EPEA) at 5 mg / mL (the drug was purchased from SIGMA); slowly add 126.6 mL of water, mix the mixed solution evenly, and finally add 7.5mL of organosilicon solution was stirred evenly to obtain the preparation.

[0047] Get 100 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is five leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control. The Botrytis cinerea pathogen was sprayed and inoculated on the plants treated with the preparation and the control, so that the suspension of the pathogen was evenly distributed on the leaves, and the inoculation concentration of the Botrytis cinerea pathogen was 2×10 5 Spores / ml. All the plants treated above were placed at 25°C, 95% humidity, 12h lig...

Embodiment 3

[0050] Measure 207.3 mL of ethanol solution of eicosapentaenoic acid ethanolamine (EPEA) at 5 mg / mL (the drug is purchased from SIGMA); slowly add 83.7 mL of water, mix the mixed solution evenly, and finally add 9.0 mL of organosilicon solution was stirred evenly to obtain the preparation.

[0051] Choose two adjacent plastic greenhouses whose cultivation technology and management are completely consistent. In the rainy season at the end of spring, use the preparation of the present invention for the treatment shed. Get 200 milliliters of the preparation of the present invention, add 20 liters of clear water and mix evenly, and divide it in the morning and evening. Spray evenly twice on the surface of tomato leaves with a seedling age of seven leaves and one heart until the leaves are wet, and continue spraying for 2 days. The control booths were only sprayed with the same volume of water. Ten days later, the disease incidence of 60 tomato plants in the treatment shed and the...

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Abstract

The present invention discloses applications of eicosapentaenoylethanolamide in improvement of plant gray mold resistance and in preparation of plant gray mold resistance improving preparations. According to the present invention, eicosapentaenoylethanolamide is adopted as a main active ingredient to prepare the preparation, and the signal paths of jasmonic acid and ethylene in plants are induced so as to significantly enhance the gray mold resistance of the plant and reduce the economic loss of the gray mold on the plant; and with the application of the preparation to prevent and control the plant gray mold, the advantages of simpleness, easy performing and low cost are provided, the growth of the Botrytis cinerea pathogenic bacteria on the leaves and the plant disease diffusion can be significantly delayed and inhibited, and the gray mold resistance of the plant is substantially improved.

Description

technical field [0001] The invention relates to the field of phytochemical protection, in particular to the application of docosapentaenoic acid ethanolamine in improving the resistance of plants to botrytis cinerea. Background technique [0002] Botrytis cinerea caused by Botrytis cinerea is a worldwide disease. Under suitable conditions such as low temperature and high humidity, the germinated conidia spread with airflow, irrigation water and agricultural tools. and infects from host wounds or dead tissue. Botrytis cinerea is a typical epidemic disease, with a short incubation period, rapid prevalence and intermittent outbreaks, and a wide range of hosts, especially on tomatoes. Botrytis cinerea can infect tomato leaves, stems, flowers, fruits and other tissues, resulting in severe reduction in yield and income, or even no harvest. It has also become a restrictive obstacle to tomato production in my country and even in the world. In addition to tomatoes, Botrytis cinerea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/20A01P21/00A01P3/00
Inventor 师恺史军营喻景权邵淑君吴爱芳
Owner ZHEJIANG UNIV
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