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Applications of 15,16-dihydrotanshinone I in preparation of medicines protecting blood vessel endothelia

A technology for dihydrotanshinone to protect blood vessels, which can be used in drug combinations, cardiovascular system diseases, pharmaceutical formulations, etc., and can solve problems such as less research on individual pharmacological activities

Inactive Publication Date: 2016-01-27
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

15,16-Dihydrotanshinone I is one of the active ingredients, but there are few studies on its single pharmacological activity

Method used

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  • Applications of 15,16-dihydrotanshinone I in preparation of medicines protecting blood vessel endothelia
  • Applications of 15,16-dihydrotanshinone I in preparation of medicines protecting blood vessel endothelia
  • Applications of 15,16-dihydrotanshinone I in preparation of medicines protecting blood vessel endothelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Preparation of 15,16-dihydrotanshinone I compound

[0013] 1 Materials and Instruments

[0014] Column chromatography silica gel (100-200 mesh, 200-300 mesh) and thin-layer chromatography silica gel H were purchased from Qingdao Ocean Chemical Co., Ltd. (chemically pure). Reagents for extraction and separation petroleum ether (Wuxi Yasheng), ethyl acetate (Nanjing Chemical Reagents), chloroform (Shanghai Lingfeng), methanol (Jiangsu Hanbang), acetone (Nanjing Chemical Reagents) are all analytically pure, and identification reagents methanol (Jiangsu Hanbang), acetonitrile (Merck) are chromatographically pure. Tanshinone Extract: Purchased from Shandong Huaxin Pharmaceutical Co., Ltd., the total tanshinone content is about 8%.

[0015] Agilent1100 liquid chromatography-time-of-flight mass spectrometry system (Agilent Company); medium pressure preparation system (Switzerland) company); rotary evaporator RotavaporR-200 (Switzerland company).

[0016]...

experiment example 2

[0026] Experimental example 2: Detection of phenotypic indicators of the effect of 15,16-dihydrotanshinone I on vascular endothelial cells (MTT, LDH)

[0027] 1 Materials and Instruments

[0028] EA.hy926 cells: purchased from ATCC and preserved by our laboratory. DMEM high glucose medium (ATCC company), fetal bovine serum, 0.25% trypsin, penicillin 10kU / mL, streptomycin 10kU / mL (Invitrogen company), PBS (Beijing Boaosen Biotechnology Co., Ltd.), RIPA lysate , oxidized low-density lipoprotein (ox-LDL) (Beijing Xiesheng Biotechnology Co., Ltd.), PhosSTOP, completeULTRATablets, MiniEASYpack (Roche Company), MTT (BioSharp Company), lactate dehydrogenase (LDH) kit (Nanjing Jiancheng Institute of Bioengineering).

[0029] Ultra-clean bench (Thermo Scientific 1300A2, Thermo Scientific, USA), microplate reader (Biotek Synergy 2), electric constant temperature water bath (Guohua Electric Co., Ltd., HH-4 digital display constant temperature water bath), CO 2 Incubator (Thermo Compan...

experiment example 3

[0045] Experimental example 3: Enzyme function level detection (SOD) experiment of the effect of 15,16-dihydrotanshinone Ⅰ on vascular endothelial cells

[0046] 1 Materials and Instruments

[0047] CompleteULTRATablets, Mini, EASYpack (Roche Company), M-PER mammalian cell total protein extraction reagent, Halt protease and phosphatase inhibitor mixture (100X) (Thermo Fisher Scientific). For the rest of the reagents, refer to Part 1 of Experimental Example 1.

[0048] Ultra-clean bench (Thermo Scientific 1300A2, Thermo Scientific, USA), microplate reader (Biotek Synergy 2), electric constant temperature water bath (Guohua Electric Co., Ltd., HH-4 digital display constant temperature water bath), CO 2 Incubator (Thermo Company of the United States, series2WaterJacket), centrifuge (Thermo Company of the United States, Fresco17), balance (Beijing Sartorius Scientific Instrument Co., Ltd., BT224S), cell culture consumables, micropipettes: Thermo Company of the United States.

[...

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Abstract

The invention relates to applications of a compound in preparation of medicines protecting blood vessel endothelia and anti-oxidative medicines, and belongs to the natural medicine field. The invention provides a compound protecting endothelial cells from oxidative damage caused by ox-LDL. The compound is 15,16-dihydrotanshinone I which is extracted from salvia miltiorrhiza. 15,16-dihydrotanshinone I can improve decrease of the cell survival rate of EA.hy926 and SOD enzyme activity in cells caused by ox-LDL stimulation, and can activate ARE elements in the Nrf2 downstream. The function of protecting endothelial cells may be achieved through inducible expression of a plurality of antioxidant enzymes after activation of Nrf2-ARE passage. A new mean is provided for 15,16-dihydrotanshinone I in treating cardiovascular diseases.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to the use of a compound in the preparation of vascular endothelial protection medicines. Background technique [0002] In recent years, the morbidity and mortality of cardiovascular diseases in the Chinese population have been increasing. At present, it is estimated that there are about 290 million people with cardiovascular diseases, and the age of onset is earlier. It can be seen that cardiovascular disease has seriously threatened the life and health of human beings today. Cardiovascular diseases include atherosclerosis, hypertension, etc. The etiology and pathogenesis are complex and diverse, which brings difficulties to treatment. Compared with western medicine, traditional Chinese medicine has definite curative effect and less toxic and side effects, and has thousands of years of clinical experience. The discovery and preparation of prodrugs from traditional Chinese medicin...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61P9/14A61P7/02A61P9/12A61P9/10A61P9/00A61P39/06
Inventor 陈君李萍张柳周萍张慧
Owner CHINA PHARM UNIV
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