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Trichosanthes kirilowii yellow pigment extraction technology

A technology of yellow pigment and Trichosanthes, which is applied in the field of extraction and preparation of natural plant pigments, can solve the problems of low extraction rate, difficult dissolution and extraction of Trichosanthes yellow pigment, and achieve the effects of easy extraction, easy filtration, and reduced viscosity

Inactive Publication Date: 2016-01-27
周子童
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that Trichomonas yellow pigment is difficult to dissolve, difficult to extract, and the extraction rate is low, the present invention provides an extraction process of Trichosanthes yellow pigment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take Trichosanthes, remove seeds and skins, put Trichosanthes pulp into a food refiner and pulverize to obtain a feed liquid, weigh 10g of the feed liquid, add 150ml of deionized water, mix, and then use a mass concentration of 10% lemon Adjust the acid to pH = 4.0 to obtain a mixture, then add 0.05g of pectinase and 0.1g of cellulase, enzymatically hydrolyze for 3 hours at 60°C, vacuum filter, the filter cake is the pigment part, add 200ml Dissolve ethanol with a mass concentration of 95%, put it in a water bath, heat at 60°C for 90 minutes, filter to remove impurities, take 1ml of the filtrate, dilute it to 25ml with 95% ethanol, and measure its absorbance at 438nm A =0.72, after the remaining filtrate was concentrated under reduced pressure to recover ethanol, it was vacuum-dried to obtain 1.1g of finished product, and the primary extraction rate was 11%.

[0015] The enzyme activity of the pectinase is 50,000 u / g.

[0016] The enzyme activity of the pectinase is 10...

Embodiment 2

[0018] Take Trichosanthes, peel, put Trichosanthes pulp into a food refiner and pulverize to obtain a feed liquid, weigh 10g of the feed liquid, add 150ml deionized water, mix, and then adjust to 10% citric acid with a mass concentration of pH value = 5.3, get the mixture, then add 0.03g pectinase and 0.07g cellulase, enzymolysis under the condition of 60 ℃ for 3 hours, vacuum filtration, the filter cake is the pigment part, add 200ml mass concentration Dissolve 95% ethanol, put it in a water bath, heat at 50°C for 90 minutes, filter to remove impurities, take 1ml of the filtrate, dilute it to 25ml with 95% ethanol, measure its absorbance at 438nm, A=0.65, The remaining filtrate was concentrated under reduced pressure to recover ethanol, and then vacuum-dried to obtain 0.89 g of the finished product, with a primary extraction rate of 8.9%.

[0019] The enzyme activity of the pectinase is 50,000 u / g.

[0020] The enzyme activity of the pectinase is 10,000 u / g.

Embodiment 3

[0022] Take Trichosanthes, remove seeds and skins, put Trichosanthes pulp into a food refiner and pulverize to obtain a feed liquid, weigh 10g of the feed liquid, add 150ml of deionized water, mix, and then use a mass concentration of 10% lemon Adjust the pH value to 6.0 with acid, then add 0.01g pectinase and 0.03g cellulase, enzymatically hydrolyze for 3 hours at 55°C, vacuum filter, the filter cake is the pigment part, add 200ml mass concentration of 95 % ethanol, put it in a water bath, heat at 60°C for 90 minutes, filter to remove impurities, filter, take 1ml of the filtrate, dilute it to 25ml with 95% ethanol, and measure its absorbance at 438nm A=0.66 , the remaining filtrate was concentrated under reduced pressure, and dried in vacuo to obtain 0.92 g of the finished product, with a primary extraction rate of 9.2%.

[0023] The enzyme activity of the pectinase is 50,000 u / g.

[0024] The enzyme activity of the pectinase is 10,000 u / g.

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Abstract

The present invention relates to a trichosanthes kirilowii yellow pigment extraction technology to solve the problems of being insoluble, different to extract and low in extraction efficiency of trichosanthes kirilowii yellow pigment, the trichosanthes kirilowii is taken, seeded and peeled, trichosanthes kirilowii pulp is crushed, added with deionized water for mixing, pH is adjusted with citric acid, pectinase and cellulase are added for enzymatic hydrolysis, filter cake is obtained by vacuum filtration, the filter cake is added with ethanol and dissolved, then put in 50 DEG C water bath for 90 minutes, and filtered to obtain filtrate, and the filtrate is concentrated under reduced pressure and is dried in vacuum to obtain the trichosanthes kirilowii yellow pigment. A dual enzymatic method is used for destruction of pectin and cellulose cell wall for release of intracellular yellow pigment, so that the extraction is easy, the yield is improved, the viscosity of the extract can be reduced, and the extract is easy to filter.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of natural plant pigments, and in particular relates to an extraction process of trichosanthes yellow pigment. Background technique [0002] Trichosanthes is a perennial herbaceous vine of Cucurbitaceae, distributed in most parts of the country. The yellow pigment containing lutein and carotene in ripe fruit has strong antioxidant capacity, and it is also the raw material of drugs for treating eye fundus lesions. The research and development of natural, safe and multifunctional pigments is the development of the food and pharmaceutical industries. direction. [0003] Castanopsis has been widely planted in my country from ancient times to the present, and its fruits, seeds and roots can be used alone for medicinal purposes. The skin of the ripe fruit is sweet in taste and cold in nature, and has the effects of nourishing the lungs and resolving phlegm, reducing fire and relievi...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
Inventor 周子童
Owner 周子童
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