Anti-nuclear polyhedrosis SNP molecular markers for silkworm and application thereof

A molecular marker and anti-blood technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problem of large error in experimental results and achieve the effect of improving efficiency

Active Publication Date: 2016-02-03
JIANGSU UNIV OF SCI & TECH
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  • Description
  • Claims
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Problems solved by technology

Due to the influence of various conditions such as virus (freshness, purity), tem...

Method used

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  • Anti-nuclear polyhedrosis SNP molecular markers for silkworm and application thereof
  • Anti-nuclear polyhedrosis SNP molecular markers for silkworm and application thereof
  • Anti-nuclear polyhedrosis SNP molecular markers for silkworm and application thereof

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Experimental program
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Embodiment 1

[0016] 1. Acquisition of cultivar-specific SNP molecular markers resistant to pus

[0017] Based on SLAF-seq technology, degenerate genome sequencing was performed on 12 different silkworm varieties (6 resistant varieties and 6 non-resistant varieties), and a total of more than 10,000 SNP sites were obtained, which were distributed in 28 linkages of silkworm. on the group. Analyzing all the SNP markers, it was found that on all linkage groups except the 27th linkage group (chr27), the SNP markers were irregularly found in resistant varieties and non-resistant varieties, and 50 SNP markers were obtained on chr27, these The SNP markers present exactly the same bases in all resistant varieties, but different bases in non-resistant varieties.

[0018]

[0019]

[0020] 2. SNP polymorphism primer design

[0021] According to the published genome information of chr27 in the fine map of silkworm genome, gene chip and SNP linkage map and other data resources [Bombyx mori genom...

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Abstract

The invention relates to anti-nuclear polyhedrosis SNP molecular markers for silkworm and application thereof. The SNPs are on a 27th linkage group and are respectively chr27-10850279-A, chr27-13227410-G and chr27-13560821-C. Silkworm-based SNP markers obtained through genomic sequencing are developed, an SNP fingerprint spectrum for a silkworm anti-nuclear polyhedrosis variety is constructed, the silkworm anti-nuclear polyhedrosis variety can be detected and screened by utilizing combinations of the SNP markers, and anti-nuclear polyhedrosis silkworm breeding efficiency is increased.

Description

technical field [0001] The invention relates to a SNP molecular marker of silkworm anti-blood type pus and its application. Background technique [0002] Bombyx mori blood-type pustosis is caused by Bombyxmori Nuclear Polyhedrosis Virus (BmNPV), which is the most serious disease to sericulture production. According to statistics, the annual loss of the disease to the sericulture industry accounts for more than 50% of the total loss of silkworm diseases, and in some places it is as high as 80%, and there is even a serious situation of no harvest. The applicant screened and obtained a special germplasm material with high resistance to BmNPV from hundreds of germplasm resources. 9 PIB / ml is 3 to 4 orders of magnitude higher than that of conventional varieties, and its disease resistance is controlled by dominant dominant genes through genetic analysis. To this end, we designed to hybridize the carrier variety with the main disease-resistant gene with the current practical sil...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 徐安英钱荷英李刚李龙孙平江张月华刘明珠
Owner JIANGSU UNIV OF SCI & TECH
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