Detection reagent, application thereof and kit containing reagent
A technology for detecting reagents and kits, which is applied in biological testing, material inspection products, etc., can solve the problems of low sensitivity and poor stability of heparin, and achieve the effects of good stability, good source of reagents, and cost savings
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Embodiment 1
[0029] 1. Weigh 1-20 grams of kaolin and dissolve it in 100ml of deionized water. After stirring for 2-4 hours, stand still at 15-35°C for 5-15 hours, and set aside.
[0030] 2. Add 100ml of the above suspension to 900ml (0.05-2g) Tris-HCL buffer solution, hepes buffer solution, phosphate buffer solution or borate buffer solution with a pH of 7.0, and stir for 30-60min , until the whole liquid presents a uniform white suspension, then add 0.2~1mg cephalin, 0.1~1g dextran 4w, 0.1~1.0g sodium azide, 1~20gBSA, 4~40gGly, stir for 20~60min and mix well. This liquid is the APTT reagent.
Embodiment 2
[0032] 1. Weigh 1g of kaolin and dissolve it in 100ml of deionized water. After stirring for 2-4 hours, let it rest at 15-35°C for 5-15 hours, and set aside.
[0033] 2. Add 100ml of the above suspended suspension into 900ml of 80mmol / l Tris-HCL buffer solution with a pH of 7.0 (corresponding to Tris-HCL0.5g), and stir for 30-60min until the whole liquid presents a uniform white mixture. Suspension, then add 0.6mg rabbit cephalin, 0.1g dextran 4w, 0.1g sodium azide, 1gBSA, 40gGly, stir for 20-60min and mix well, the liquid is APTT reagent.
Embodiment 3
[0035] 1. Weigh 20g of kaolin and dissolve it in 100ml of deionized water. After stirring for 2-4 hours, let it rest at 15-35°C for 5-15 hours, and set aside.
[0036] 2. Add 100ml of the above-mentioned static suspension into 900ml of 300mmol / L Tris-HCL buffer solution (corresponding to Tris-HCL 2g) with a pH of 7.0, and stir for 30-60min until the whole liquid presents a uniform white suspension , then add 0.2mg porcine cephalin, 1g dextran 4w, 0.5g sodium azide, 1gBSA, 22gGly and stir for 20-60min to mix well, the liquid is the APTT reagent.
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