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Detection reagent and its application and kit containing the reagent

A technology for detecting reagents and buffers, which is applied in biological testing and material inspection products, can solve the problems of poor stability and low sensitivity of heparin, and achieve the effects of good reagent source, good stability and cost saving

Active Publication Date: 2018-04-10
BEIJING MDC NEW SPRING MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid APTT reagents commonly used in the existing market mostly use ellagic acid as an activator, which is less sensitive to heparin and has poor stability

Method used

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  • Detection reagent and its application and kit containing the reagent
  • Detection reagent and its application and kit containing the reagent
  • Detection reagent and its application and kit containing the reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Weigh 1-20 grams of kaolin and dissolve it in 100ml of deionized water. After stirring for 2-4 hours, stand still at 15-35°C for 5-15 hours, and set aside.

[0030] 2. Add 100ml of the above suspension to 900ml (0.05-2g) Tris-HCL buffer solution, hepes buffer solution, phosphate buffer solution or borate buffer solution with a pH of 7.0, and stir for 30-60min , until the whole liquid presents a uniform white suspension, then add 0.2~1mg cephalin, 0.1~1g dextran 4w, 0.1~1.0g sodium azide, 1~20g BSA, 4~40g Gly, stir for 20~60min and mix well , the liquid is the APTT reagent.

Embodiment 2

[0032] 1. Weigh 1g of kaolin and dissolve it in 100ml of deionized water. After stirring for 2-4 hours, let it rest at 15-35°C for 5-15 hours, and set aside.

[0033] 2. Add 100ml of the above-mentioned suspended suspension into 900ml of 80mmol / l Tris-HCL buffer solution with a pH of 7.0 (corresponding to Tris-HCL0.5g), and stir for 30-60min until the whole liquid appears uniform white Then add 0.6mg rabbit cephalin, 0.1g dextran 4w, 0.1g sodium azide, 1g BSA, 40g Gly and stir for 20-60min to mix well. The liquid is the APTT reagent.

Embodiment 3

[0035] 1. Weigh 20g of kaolin and dissolve it in 100ml of deionized water. After stirring for 2-4 hours, let it rest at 15-35°C for 5-15 hours, and set aside.

[0036] 2. Add 100ml of the above-mentioned suspended suspension to 900ml of 300mmol / L Tris-HCL buffer solution (corresponding to Tris-HCL 2g) with a pH of 7.0, and stir for 30-60min until the whole liquid presents a uniform white suspension Then add 0.2mg porcine cephalin, 1g dextran 4w, 0.5g sodium azide, 1g BSA, 22g Gly and stir for 20-60min to mix well. The liquid is the APTT reagent.

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PUM

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Abstract

The invention relates to the technical field of biology, and especially relates to an activated partial thromboplastin time (APTT) detection reagent, application thereof and a kit containing the reagent. The detection reagent comprises kaolin, cephalin, a buffer, bovine serum albumin (BSA), glycine, glucan and NaN3. Compared with the prior art, the technical scheme comprises the following advantages: by adjusting the compositions and the proportions of a protective solution of the APTT kit, the APTT reagent stability is extremely good, is stable within 18 months at 4 DEG C, is stable within 15 days at 37 DEG C, and is stable within 50 days at 4 DEG C after being opened. The product stability is substantially (P<0.05) better than common commercially-available products, cost is saved for hospitals and extremely good reagent source is provided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a reagent for measuring activated partial thromboplastin time (APTT), an application thereof and a kit containing the reagent. Background technique [0002] In terms of clinical testing, the various departments of the hospital have gained a wide understanding of the detection of hemostasis and thrombus. The detection of hemostasis and thrombus has provided clinicians with sufficient support for disease diagnosis, observation of curative effect, detection of anticoagulant dosage and prognosis. Supported by a large amount of reliable data, as people in China understand and pay more attention to this aspect, more and more detection methods are used in it. [0003] The coagulation pathway of the human body is divided into the intrinsic coagulation pathway and the extrinsic coagulation pathway, and the activated partial thromboplastin time (APTT) is an important indicator for detecting wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 王争胡琦胡军
Owner BEIJING MDC NEW SPRING MEDICAL DEVICES
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