Fry preparation method for all-male takifugu rubripus and application

A technology of puffer and male fish, which is applied in the fields of application, climate change adaptation, fish farming, etc., can solve the problems of poisonous puffer, food restriction, hindering development and utilization, etc., and achieves huge application potential and market promising effect

Inactive Publication Date: 2016-03-02
YANTAI DEV ZONE TIANYUAN AQUATIC PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The breeding technology of pufferfish seedlings in my country was successfully researched and developed by the Yellow Sea Fisheries Research Institu

Method used

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  • Fry preparation method for all-male takifugu rubripus and application
  • Fry preparation method for all-male takifugu rubripus and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] 1. Using estrogen to induce the sexual transformation of the original line of redfin puffer fish into functional female fish of the transformed line

[0039] (1) Preparation of estrogen feed

[0040] Mix 100 mg of estradiol benzoate as estrogen with 100 mL of medical 100% alcohol, and then add 1 kg of juvenile fish feed to mix evenly, configure it into a 100 mg / kg feed, and spread it out at room temperature for 24 hours until the ethanol evaporates. Refrigerate in bags.

[0041] (2) Induction process

[0042]Thirty 10-day-old male redfin puffer fish (XY, ♂) were selected and fed with 100 mg / kg hormone feed at 8:00 a.m. every day, and the feeding amount was 1% of their body weight. The normal feed supplemented with estrogen should not exceed 2% of the body weight per day for 60 days. By observing the body shape and cloaca characteristics with the naked eye, the male and female fish can be identified, and the functional female fish of the transformat...

Example Embodiment

[0051] Embodiment 2

[0052] 1. Using estrogen to induce the sexual transformation of the original line of redfin puffer fish into functional female fish of the transformed line

[0053] (1) Preparation of estrogen feed

[0054] Mix 200 mg of ethinyl estradiol as estrogen with 200 mL of medical 100% alcohol, then add 1 kg of juvenile fish feed and mix evenly, configure it into a 200 mg / kg feed, and spread it at room temperature for 24 hours until the ethanol evaporates. Refrigerate in bags.

[0055] (2) Induction process

[0056] Thirty 10-day-old male redfin puffer fish (XY, ♂) were selected and fed 200 mg / kg hormone feed at 8:00 a.m. every day, and the feeding amount was 1.5% of their body weight. The normal feed supplemented with estrogen should not exceed 3% of the body weight per day for 60 days. By observing the body shape and cloaca characteristics with the naked eye, the male and female fish can be identified, and the functional female fish of the transformation lin...

Example Embodiment

[0060] Embodiment 3

[0061] 1. Using estrogen to induce the sexual transformation of the original line of redfin puffer fish into functional female fish of the transformed line

[0062] (1) Preparation of estrogen feed

[0063] Mix 50 mg of estradiol benzoate as estrogen with 100 mL of medical 100% alcohol, and then add 1 kg of juvenile fish feed and mix evenly, configure it into a 50 mg / kg feed, and spread it at room temperature for 24 hours until the ethanol evaporates. Refrigerate in bags.

[0064] (2) Induction process

[0065] Thirty 10-day-old males (XY, ♂) were selected and fed 50 mg / kg hormone feed at 8:00 a.m. every day, and the feeding amount was 1% of their body weight. The normal feed supplemented with estrogen should not exceed 2.5% of the body weight per day for 60 days. By observing the body shape and cloaca characteristics with the naked eye, the male and female fish can be identified, and the functional female fish of the transformed line can be obtained (...

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Abstract

The invention relates to the breeding field of marine fishes and particularly relates to a fry preparation method for all-male takifugu rubripus. The method comprises the following steps of: (1) inducing sex transformation of original line male takifugu rubripus by using a female takifugu rubripus hormone to transformed line functional female takifugu rubripus; (2) performing mating on the transformed line functional female takifugu rubripus and normal male takifugu rubripus to obtain supermale takifugu rubripus; and (3) performing mating on the supermale takifugu rubripus and normal female takifugu rubripus to obtain fries, wherein all genotypes are male. According to the takifugu rubripus fries produced by virtue of the fry preparation method provided by the invention, all genotypes are male (XY, male), the survival rate of all-male takifugu rubripus can reach over 90%, and 100% fries with phenotypes of being male can be obtained if being cultured under a certain environmental condition. The preparation method is wide in market prospect and huge in application potential.

Description

technical field [0001] The invention relates to the field of seawater fish cultivation, in particular to a seed production method and application of all male red-finned pufferfish. Background technique [0002] Redfin puffer (Takifugurubripus) is one of the fish with the highest economic value, and it is also one of the important export-earning aquatic products in my country. It is famous for its delicious meat and various eating methods. It is popular in Japan, Korea and my country Pufferfish have a long history, and there has long been a saying that "eat pufferfish desperately". Especially in Japan, the red-finned pufferfish is regarded as "the king of fish" and "the head of a hundred fish", and its sashimi is the most popular , the price is expensive, and has always been an important representative of Japanese food culture; Tetrodotoxin (TTX) and its preparations contained in the ovary, liver, blood, intestine, kidney, spinal cord, spleen, etc. The high-grade drug of the dr...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 曲江波刘新富孟振徐荣静
Owner YANTAI DEV ZONE TIANYUAN AQUATIC PROD CO LTD
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