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Fry preparation method for all-male takifugu rubripus and application

A technology of puffer and male fish, which is applied in the fields of application, climate change adaptation, fish farming, etc., can solve the problems of poisonous puffer, food restriction, hindering development and utilization, etc., and achieves huge application potential and market promising effect

Inactive Publication Date: 2016-03-02
YANTAI DEV ZONE TIANYUAN AQUATIC PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The breeding technology of pufferfish seedlings in my country was successfully researched and developed by the Yellow Sea Fisheries Research Institute in the early 1990s. However, because pufferfish is poisonous, its consumption in my country is restricted, which hinders its development and utilization.

Method used

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  • Fry preparation method for all-male takifugu rubripus and application
  • Fry preparation method for all-male takifugu rubripus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Using estrogen to induce the sex transformation of the original redfin puffer fish into the functional female fish of the transformed line

[0039] (1) Preparation of estrogen feed

[0040] Mix 100mg of estradiol benzoate as estrogen with 100mL of medical 100% alcohol, then add 1kg of juvenile fish feed and mix evenly, configure 100mg / kg feed, and spread it at room temperature for 24 hours until the ethanol volatilizes. Store in bags and refrigerate.

[0041] (2) Induction process

[0042]Select 30 redfin puffer males (XY, ♂) at the age of 10 days, and feed them 100 mg / kg hormone feed at 8:00 every day at 1% of their body weight. The normal feed with estrogen added, the total daily feeding amount does not exceed 2% of the body weight, and lasts for 60 days. The male and female can be identified by visually observing the body shape and cloaca characteristics, and the transformed female fish (XY, ♀) can be obtained. , and the conversion rate was 83.3%.

[0043] 2. ...

Embodiment 2

[0052] 1. Using estrogen to induce the sex transformation of the original redfin puffer fish into the functional female fish of the transformed line

[0053] (1) Preparation of estrogen feed

[0054] Mix 200mg of ethinyl estradiol as estrogen with 200mL of medical 100% alcohol, then add 1kg of juvenile fish feed and mix evenly, configure 200mg / kg feed, and spread it at room temperature for 24 hours until the ethanol volatilizes. Store in bags and refrigerate.

[0055] (2) Induction process

[0056] Select 30 redfin puffer males (XY, ♂) at the age of 10 days, and feed them 200 mg / kg hormone feed at 8:00 every day at 1.5% of their body weight. The normal feed with estrogen added, the total daily feeding amount does not exceed 3% of the body weight, and lasts for 60 days. By visually observing the body shape and cloaca characteristics, the male and female can be identified, and the transformed female fish (XY, ♀) can be obtained. , and the conversion rate was 90.0%.

[0057] ...

Embodiment 3

[0061] 1. Using estrogen to induce the sex transformation of the original redfin puffer fish into the functional female fish of the transformed line

[0062] (1) Preparation of estrogen feed

[0063] Mix 50mg of estradiol benzoate as estrogen with 100mL of medical 100% alcohol, then add 1kg of juvenile fish feed and mix evenly, configure 50mg / kg feed, and spread it at room temperature for 24 hours until the ethanol volatilizes. Store in bags and refrigerate.

[0064] (2) Induction process

[0065] 30 10-day-old redfin puffer males (XY, ♂) were selected and fed with 50 mg / kg hormone feed at 8:00 am every day at 1% of body weight. The normal feed with estrogen added, the total daily feeding amount does not exceed 2.5% of the body weight, and lasts for 60 days. The male and female can be identified by visually observing the body shape and cloaca characteristics, and the transformed female fish (XY, ♀) can be obtained. , and the conversion rate was 76.6%.

[0066] 2. According...

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Abstract

The invention relates to the breeding field of marine fishes and particularly relates to a fry preparation method for all-male takifugu rubripus. The method comprises the following steps of: (1) inducing sex transformation of original line male takifugu rubripus by using a female takifugu rubripus hormone to transformed line functional female takifugu rubripus; (2) performing mating on the transformed line functional female takifugu rubripus and normal male takifugu rubripus to obtain supermale takifugu rubripus; and (3) performing mating on the supermale takifugu rubripus and normal female takifugu rubripus to obtain fries, wherein all genotypes are male. According to the takifugu rubripus fries produced by virtue of the fry preparation method provided by the invention, all genotypes are male (XY, male), the survival rate of all-male takifugu rubripus can reach over 90%, and 100% fries with phenotypes of being male can be obtained if being cultured under a certain environmental condition. The preparation method is wide in market prospect and huge in application potential.

Description

technical field [0001] The invention relates to the field of seawater fish cultivation, in particular to a seed production method and application of all male red-finned pufferfish. Background technique [0002] Redfin puffer (Takifugurubripus) is one of the fish with the highest economic value, and it is also one of the important export-earning aquatic products in my country. It is famous for its delicious meat and various eating methods. It is popular in Japan, Korea and my country Pufferfish have a long history, and there has long been a saying that "eat pufferfish desperately". Especially in Japan, the red-finned pufferfish is regarded as "the king of fish" and "the head of a hundred fish", and its sashimi is the most popular , the price is expensive, and has always been an important representative of Japanese food culture; Tetrodotoxin (TTX) and its preparations contained in the ovary, liver, blood, intestine, kidney, spinal cord, spleen, etc. The high-grade drug of the dr...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 曲江波刘新富孟振徐荣静
Owner YANTAI DEV ZONE TIANYUAN AQUATIC PROD CO LTD
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