A kind of preparation method of cross-linked carboxymethylated agarose-based gel microspheres
A technology for cross-linking carboxymethyl and gel microspheres, applied in chemical instruments and methods, inorganic chemistry, other chemical processes, etc., can solve the problem of affecting the separation performance and mechanical strength of carboxymethylated agarose-based gel microspheres , The mechanical strength and pore size structure of cross-linked microspheres are easy to change, and the mechanical strength and pore size of microspheres are not easy to control, so as to achieve good application prospects, stability assurance, and easy control.
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Embodiment 1
[0019] (1) In a 500ml three-necked flask, add 25g of agarose, 150ml of 95% ethanol and 40g of NaOH, stir at room temperature for 12h, then slowly add 25g of chloroacetic acid (dissolve it in 50ml of 95% ethanol) dropwise, and keep the reaction The temperature of the liquid is not higher than 45°C, and it takes about 90 minutes. Then the temperature was raised to 65°C for 8 hours. After completion, filter with filter paper, and the collected precipitate was washed with 95% ethanol and dried at 80°C for more than 24 hours to obtain carboxymethylated agarose;
[0020] (2) Weigh 4g of carboxymethylated agarose, add it into 100ml of water and heat to dissolve, make a carboxymethylated agarose solution with a concentration of 4% (g / ml), keep it warm at 90°C for subsequent use;
[0021] (3) In a 1000ml three-neck flask, add 500ml 6% (v / v) soybean oil of Span 80, 800r min -1 Stir at a rotating speed, heat up to 90°C, then add 100ml of 90°C 4% carboxymethylated agarose (prepared in st...
Embodiment 2
[0032] (1) In a 500ml three-necked flask, add 25g of agarose, 150ml of 95% ethanol and 40g of NaOH, stir at room temperature for 12h, then slowly add 25g of chloroacetic acid (dissolve it in 50ml of 95% ethanol) dropwise, and keep the reaction The temperature of the liquid is not higher than 45°C, and it takes about 90 minutes. Then the temperature was raised to 65°C for 8 hours. After completion, filter with filter paper, and the collected precipitate was washed with 95% ethanol and dried at 80°C for more than 24 hours to obtain carboxymethylated agarose;
[0033] (2) Weigh 4g of carboxymethylated agarose, add it into 100ml of water and heat to dissolve, make a carboxymethylated agarose solution with a concentration of 4% (g / ml), keep it warm at 90°C for subsequent use;
[0034] (3) In a 1000ml three-necked flask, add the soybean oil of 500ml 6% Span 80, stir at a rotating speed of 800r min-1, heat up to 90°C, then add 4% carboxymethylated agarose of 100ml 90°C (step ( 2) Pr...
Embodiment 3
[0043] (1) In a 500ml three-necked flask, add 25g of agarose, 150ml of 95% ethanol and 40g of NaOH, stir at room temperature for 12h, then slowly add 25g of chloroacetic acid (dissolve it in 50ml of 95% ethanol) dropwise, and keep the reaction The temperature of the liquid is not higher than 45°C, and it takes about 90 minutes. Then the temperature was raised to 65°C for 8 hours. After completion, filter with filter paper, and the collected precipitate was washed with 95% ethanol and dried at 80°C for more than 24 hours to obtain carboxymethylated agarose;
[0044] (2) Weigh 4g of carboxymethylated agarose, add it into 100ml of water and heat to dissolve, make a carboxymethylated agarose solution with a concentration of 4% (g / ml), keep it warm at 90°C for subsequent use;
[0045] (3) In a 1000ml three-necked flask, add the soybean oil of 500ml 6% Span 80, stir at a rotating speed of 800r min-1, heat up to 90°C, then add 4% carboxymethylated agarose of 100ml 90°C (step ( 2) Pr...
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