A method for analyzing powder flavor samples using leaching method
A powder essence and sample technology, which is applied in the field of analysis of powder essence samples by leaching method, can solve the problems of difficulty in collecting aroma, low content of raw materials, easy volatility, etc., and achieve the effect of improving test efficiency, good reproducibility, and easy operation
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Embodiment 1
[0018] (1) Mix the powder essence sample with acetone in a mass-to-volume ratio of 1g:1ml, stir with a stirrer at medium speed for 60 minutes, stand at 0°C for 4 hours to get the upper layer solution, filter with filter paper, and then use the leaching solution for 3000 rpm Centrifuge for 10 minutes and take the supernatant;
[0019] (2) Naturally volatilize the supernatant in a fume hood. When the remaining amount is greater than 2-3 mL, stop the volatilization, take out the remaining solution, and perform GC-MS injection analysis;
[0020] (3) Repeat the extraction and analysis of steps (1) to (2) with ether, and summarize the two analysis results to obtain the sample analysis results. see the results figure 1 and figure 2 .
[0021] The chromatographic conditions used for GC-MS are as follows:
[0022] Chromatographic column: 19091J-433, HP-5;
[0023] 30m*0.25mm*0.25μm;
[0024] Carrier gas: helium, 35cm / sec, 0.9ml / min;
[0025] Column oven: 50℃, keep for 1min;
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Embodiment 2
[0032] (1) Mix the powdered essence sample with acetone in a mass-to-volume ratio of 1g:4ml, stir with a stirrer at medium speed for 80 minutes, stand at 2°C for 4 hours to get the upper layer solution, filter with filter paper, and then use the leaching solution for 3000 rpm Centrifuge for 20 minutes and take the supernatant;
[0033] (2) Naturally volatilize the supernatant in a fume hood, and when the remainder is greater than 2 to 3 mL, stop the volatilization, take out the remaining solution, and carry out GC-MS injection analysis; the chromatographic conditions used by GC-MS are the same as those in Example 1;
[0034] (3) Repeat the extraction and analysis of steps (1) to (2) with ether, and summarize the two analysis results to obtain the sample analysis results.
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