Method for detecting the content of mold and yeast in samples
A technology for detecting samples and yeasts, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms, etc., can solve problems such as the inability to detect the content of molds and yeasts, and achieve high accuracy
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Embodiment 1
[0014] The instruments, materials and operating methods for detecting the content of mold and yeast in gum or jam substances are as follows:
[0015] 1. Experimental equipment
[0016] Constant temperature incubator: 28℃±1℃;
[0017] Refrigerator: 2℃~5℃;
[0018] Balance: Sensitivity is 0.1g;
[0019] homogenizer.
[0020] 2. Experimental reagents and materials
[0021] Sterile pipette: 1mL (with 0.01mL scale), 10mL (with 0.1mL scale) or micropipette and tip;
[0022] Sterile Erlenmeyer flask: capacity 250mL, 500mL;
[0023] Sterile homogenization: 90mm diameter pH meter or pH colorimetric tube or precision pH test paper;
[0024] Sterile homogeneous bag: 32cm*24cm;
[0025] slides.
[0026] 3. Preparation of culture medium
[0027] Weigh 36.6 g of Bengal red culture medium into 1000 ml of distilled water, heat to boil until completely dissolved, and autoclave at 121°C for 15 minutes for later use.
[0028] 4. Operation process
[0029] (1) Weigh 25g of sample and p...
Embodiment 2
[0050] Colony counts and reports were performed for Example 1 and Comparative Example 1 in this example.
[0051] Table 1 shows the number of colonies detected by the method of Example 1 and Comparative Example 1. As a result, it was found that the method of Comparative Example 1 could not completely detect all the bacteria. The main reason was that the glue or jam substances to be detected were mixed unevenly with the medium in the plate, and the glue or jam substances to be detected gathered into a large number of lumps. , the bacteria in it cannot contact the medium, so that they cannot grow, resulting in failure to detect. The method of Example 1 can fully detect the bacterial cells in the gum or jam substance to be detected.
[0052] The detection result of embodiment 1 and comparative example 1 is as shown in table 1,
[0053] Table 1: The total number of colonies of Example 1 and Comparative Example 1
[0054]
[0055]
[0056] As can be seen from the results in ...
Embodiment 3
[0062] Each sample to be tested was independently carried out 10 times according to the method of Example 1 and Comparative Example 2, and its mold and yeast contents were determined. As a result, it was found that when detecting according to the method of Example 1, the relative deviation between the results obtained each time was small, and when detecting according to the method of Comparative Example 2, the deviation between the results obtained each time was relatively large, and the accuracy poor.
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