Method for detecting bacteria content in raw rubber
A technology of raw materials and bacteria, which is applied in the field of detection of bacteria content in raw rubber, can solve problems that need to be improved, achieve high accuracy and improve the detection rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] In this embodiment, detect the total number of colonies in carrageenan according to the following steps:
[0036] 1. Preparation of culture medium
[0037] Nutrient broth 19.0g (commercially available);
[0038] 1% TTC solution (0.4gTTC dissolved in 40mL distilled water) 40mL;
[0039] Agar 15.0g;
[0040] Distilled water 960mL;
[0041] pH7.1~7.3(25℃).
[0042] Accurately weigh 19.0g of nutrient broth, dissolve it in 960mL of distilled water, boil it to dissolve completely, subpackage, autoclave at 121°C for 15 minutes, take it out and cool it to about 50°C, add 1% TTC that has been boiled and sterilized to every 240mL of nutrient broth Solution 10mL.
[0043] 2. Operation process
[0044] (1) Weigh 25g of carrageenan and place it in a sterile homogeneous bag containing 225mL of normal saline, perform the first homogenization treatment on the homogenizer at a speed of 10000r / min for 2min, and make a 1:10 dilution ;
[0045] (2) Weigh 10g of the diluent and plac...
Embodiment 2
[0051] According to the method of GB4789.2-2010, the colony counting and reporting of Example 1 and Comparative Example 1 were carried out.
[0052] Table 1 shows the number of colonies detected by the method of Example 1 and Comparative Example 1. As a result, it was found that the method of Comparative Example 1 could not completely detect all the bacteria. The main reason was that the raw material glue and the culture medium were mixed unevenly in the plate, and the raw material glue gathered into a large number of lumps, and the bacteria in it could not contact the culture medium, so that Unable to grow, resulting in no detection, only a small number of bacteria can be detected. The method of Example 1 can fully detect the bacteria in the raw glue.
[0053] The total number of bacterial colonies of table 1 embodiment 1 and comparative example 1
[0054]
Embodiment 3
[0062] In this embodiment, measure the detection rate of embodiment 1 and comparative example 1 method respectively according to the following steps
[0063] (1) Obtain the sample to be tested by adding target bacteria with a known number of colonies to the sterile sample;
[0064] (2) measure the colony number of the sample to be tested according to the method of embodiment 1 and comparative example 1 respectively; And
[0065] (3) Calculate the detection rate of the methods of Example 1 and Comparative Example 1 respectively, wherein the detection rate (%)=(number of colonies detected by the sample to be tested / number of colonies of known target bacteria)×100%.
[0066] Table 2 shows the detection rates of the methods of Example 1 and Comparative Example 1. It can be seen that the detection rate of the method of the present invention is relatively high, and can be used for the detection of the total number of colonies in the raw glue.
[0067] The detection rate of table 2 ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| thickness | aaaaa | aaaaa |
| thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More