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Method for detecting tropomyosin by means of homologous epitope peptide antibody

A technology of tropomyosin and myosin, which is applied in the field of detection of tropomyosin by homologous epitope peptide antibody, which can solve the problems of popularization, high research and use costs, difficulty in rapid screening of a large number of samples and on-site detection, etc. problems, to eliminate the impact of test results and solve the effect of inaccurate test results

Inactive Publication Date: 2016-03-09
OCEAN UNIV OF CHINA
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Problems solved by technology

Therefore, although detection methods such as immunoassays have achieved rapid development and have shown great potential, the high cost of research and use prevents them from being widely used as ideal rapid screening tools
For food allergens including aquatic product allergens, it is currently difficult to achieve efficient and rapid screening and on-site detection of a large number of samples, which is also one of the key and difficult issues in the field of food safety research in the world

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  • Method for detecting tropomyosin by means of homologous epitope peptide antibody
  • Method for detecting tropomyosin by means of homologous epitope peptide antibody
  • Method for detecting tropomyosin by means of homologous epitope peptide antibody

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[0027] A method for detecting tropomyosin using a homologous epitope peptide antibody, comprising the following steps:

[0028] 1) Screening of homologous epitopes of tropomyosin in seafood

[0029] Take 4 kinds of shrimp allergens with complete amino acid sequences, 4 kinds of shrimp tropomyosins and 6 kinds of crab tropomyosins, in the NCBI protein database (http: / / www.ncbi.nlm.nih.gov / protein / ) to find the corresponding sequence, and the corresponding amino acid sequence number is shown in Table 1. The amino acid sequences of the 14 proteins obtained were compared using the Clustalw software in EBI (http: / / www.ebi.ac.uk / Tools / msa / clustalw2 / ), and the results were analyzed using Bioedit software to analyze the conserved and non-conserved Regional location, screening to determine the common epitope of different tropomyosins (the standard of common epitope is the same sequence of epitope peptide), called homologous epitope.

[0030] Table 114 Accession numbers of crustacean...

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Abstract

The invention discloses a method for detecting tropomyosin by means of a homologous epitope peptide antibody. An immune analysis mode of developing detection methods one by one according to single allergens is broken through, and according to allergens of aquatic products such as shrimps and crabs, a race-specific epitope peptide antibody with broad-spectrum recognition capability is researched and prepared, a novel aquatic product allergen detection method is developed, and high-throughput rapid detection of the allergens in the aquatic products is achieved.

Description

technical field [0001] The invention relates to a method for detecting tropomyosin, in particular to a method for detecting tropomyosin by using a homologous epitope peptide antibody. Background technique [0002] With the improvement of people's living standards, the consumption of shrimp and crab aquatic products is increasing day by day. In 2013, the total output of aquatic products in the country was 59.0768 million tons, with a per capita share of 43.63 kg. It has become an important source of food for our people. However, shrimp and crab aquatic products are one of the eight major food allergens announced by the Food and Agriculture Organization of the United Nations. Research data in my country also show that shrimp and crab aquatic products are the main cause of allergic diseases among consumers. In addition, for the purpose of protecting consumer safety and trade protection, developed countries have formulated strict testing standards and indicators for food allerge...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 李振兴陆宗超林洪吕良涛
Owner OCEAN UNIV OF CHINA
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