Molecular method capable of identifying gynaikothrips ficorum and gynairothrips uzeli zimmerman

A technology for Ficus female tube thrips and Ficus tube thrips, which is applied in the field of molecular markers, can solve problems such as difficulty in accurately identifying Ficus female tube thrips and Ficus tube thrips, and achieves the effects of reducing human identification errors and lowering technical thresholds

Inactive Publication Date: 2016-03-23
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that traditional morphology is difficult to accurately identify Ficus thrips

Method used

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  • Molecular method capable of identifying gynaikothrips ficorum and gynairothrips uzeli zimmerman
  • Molecular method capable of identifying gynaikothrips ficorum and gynairothrips uzeli zimmerman
  • Molecular method capable of identifying gynaikothrips ficorum and gynairothrips uzeli zimmerman

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Experimental program
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Embodiment 1

[0060] 1 sample source

[0061] The thrips fig and thrips fig used in this example come from the collected samples of 9 populations in four provinces, and the specific collection information is shown in Table 1.

[0062] Table 1 Information on the collection of Thrips figs and Thrips figs used for the study

[0063]

[0064] 2DNA extraction

[0065] For the specific steps of extracting DNA, refer to the Blood / Cell / Tissue Genomic DNA Extraction Kit produced by Tiangen Biochemical Technology (Beijing) Co., Ltd., and some steps have been improved, as follows:

[0066] ①Pick out single-headed adults of Ficus syringus and Ficus syringus, remove the abdomen, put the rest of the tissue structure in a 1.5mL centrifuge tube, add liquid nitrogen to grind the muscles to a homogeneous state, and then add 200 μL of GA buffer liquid;

[0067] ② Add 20-40 μL proteinase K solution, shake and mix evenly, depending on the muscle mass of the insect;

[0068] ③Put it in a 56°C water bath s...

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Abstract

The invention provides a molecular method capable of identifying gynaikothrips ficorum and gynairothrips uzeli zimmerman. The molecular method comprises the following steps: (1) extracting DNA (Deoxyribose Nucleic Acid) of a sample to be identified; (2) taking the DNA extracted by the step (1) as a template, carrying out PCR (Polymerase Chain Reaction) amplification by using a primer pair and purifying an amplified product; (3) sequencing the PCR amplified product purified by the step (2), and accurately distinguishing two seeds through a basic group variation site, a genetic distance and a phylogenetic tree. The invention provides a method for identifying the gynaikothrips ficorum and the gynairothrips uzeli zimmerman through molecular biology; the method is simple to operate and accurate in identification and can be widely popularized.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a molecular method for identifying thrips fig and thrips fig. Background technique [0002] Both Gynaikothrips ficorum and Gynaikothripsuzeli belong to the genus Gynaikothrips in the family Phlaeothripidae of the order Thysanoptera, and are important species on banyan trees in tropical and subtropical regions of my country. Economic pest. Two kinds of thrips file and suck the new shoots and young leaves of the banyan tree. In the early stage, the leaves curl up and form "dumpling" or "pimple" galls. Withered symptoms, when the damage is serious, the leaves of the whole plant will turn yellow, die and fall off, especially after the bonsai banyan tree is damaged, both the ornamental value and the economic value are greatly reduced. In addition, in recent years, with the transportation of banyan seedlings and bonsai in different regions, two kinds of thrips have spread to...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6806C12Q2531/113C12Q2523/308
Inventor 张利娟崔金杰彩万志张帅雒珺瑜王春义吕丽敏李春花朱香镇王丽
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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