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An all-polarization-maintaining fiber laser lattice generation apparatus used for a stimulated emission depletion (STED) microscope

A technology of stimulated emission loss and polarization-maintaining optical fiber, which is applied in microscopes, optics, optical components, etc., can solve the problems of complex optical path, difficult adjustment of optical path construction, and large main body of optical path, so as to achieve high spot quality and facilitate integration and coupling Simple and convenient effect

Active Publication Date: 2016-04-06
NORTHWEST UNIV(CN)
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Problems solved by technology

However, using this method to realize the dot-matrix passive light-emitting spot has a large body of light path, and the light path is very complicated, which brings many difficulties to the construction of the entire STED system and the adjustment of the light path.

Method used

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  • An all-polarization-maintaining fiber laser lattice generation apparatus used for a stimulated emission depletion (STED) microscope
  • An all-polarization-maintaining fiber laser lattice generation apparatus used for a stimulated emission depletion (STED) microscope

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Embodiment Construction

[0028] Comply with the above-mentioned technical scheme, see figure 1 , the full polarization-maintaining fiber laser lattice generating device for stimulated emission loss microscope of the present invention comprises a half-wave plate 0, a coupling objective lens 1, and a polarization-maintaining fiber coupler 2 arranged in sequence along the propagation direction of the depleted excitation light , an optical fiber fixing device 4, a beam reducer 5 and a high numerical aperture objective lens 6; a plurality of polarization-maintaining optical fibers at the output end of the polarization-maintaining fiber coupler 2 are all provided with a polarization controller 3; The ends of the polarization-maintaining optical fibers all pass through the optical fiber fixing device 4; the ends of multiple polarization-maintaining optical fibers at the output end of the polarization-maintaining fiber coupler 2 all pass through the optical fiber fixing device 4, and the described optical fibe...

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Abstract

The invention discloses an all-polarization-maintaining fiber laser lattice generation apparatus used for a stimulated emission depletion (STED) microscope. The apparatus comprises a half-wave plate, a coupling object lens, a polarization maintaining fiber coupler, a fiber fixing device, a light beam compressing device and a hyper-numerical aperture object lens sequentially arranged along a deexcitation light spreading direction. A polarization controller is arranged in each of a plurality of polarization maintaining fibers at an emitting terminal of the polarization maintaining fiber coupler. The tail end of each of the plurality of polarization maintaining fibers at the emitting terminal of the polarization maintaining fiber coupler goes through the fiber fixing device. The apparatus of the present invention has advantages of a simple and compact structure, convenient adjusting, stable operation, relatively small loss, etc., and can provide a large scale latticed deexcitation laser lattice which is large in cover area and which is with a facula space about a half-wavelength of incident light and consistent shapes of laser faculas for the stimulated emission depletion (STED) microscope.

Description

technical field [0001] The invention relates to an all-polarization-maintaining fiber laser lattice generating device for a stimulated emission loss microscope. Background technique [0002] In traditional optical microscopy, due to the limitation of "Abbe's diffraction limit", the resolution limit size of the microscopic system is about half of the wavelength of the light wave used. Stimulated emission depletion (STED, stimulated emission depletion) microscopy is a new type of far-field optical microscopy technology. First, one beam of excitation light is used to excite the fluorescent molecules of the sample, and then another beam of laser light is used to suppress the fluorescence of the sample. Molecular autofluorescence radiates, thereby greatly compressing the spatial size of the fluorescent signal of the sample, and finally realizing nanometer-level resolution. Among them, the key to break through the diffraction limit of STED microscope is the depletion of excitatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B27/48G02B21/00
CPCG02B21/0004G02B27/48
Inventor 张琛白晋涛王凯歌陆宝乐王爽赵伟
Owner NORTHWEST UNIV(CN)
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