Composition for separating lymphocytes and separation liquid thereof

A lymphocyte and composition technology, applied in the field of lymphocyte separation composition and its separation liquid, can solve the problems of limited application of hydroxyethyl starch, and achieve low raw material uncertainty, high safety, and good anti-tumor activity Effect

Inactive Publication Date: 2019-01-22
北京弘润天源基因生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical application of hydroxyethyl starch is limited

Method used

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  • Composition for separating lymphocytes and separation liquid thereof
  • Composition for separating lymphocytes and separation liquid thereof
  • Composition for separating lymphocytes and separation liquid thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In an environment with local 100-level laminar flow purification conditions, at 20°C, add 1g of dextran, 4g of trehalose, 1.5g of ultra-low viscosity sodium alginate, 4g of diatrizoate, and L-methylsulfide Valine 0.006g, L-valine 0.008g and L-leucine 0.01g were fully dissolved in sterile water for injection to prepare a physiological solution 100mL, and then filtered through a 0.22μm filter membrane to obtain a sterile, endotoxin-free inside the container.

Embodiment 2

[0038] In an environment with local 100-level laminar flow purification conditions, at 20°C, dextran 4g, trehalose 6g, ultra-low viscosity sodium alginate 0.3g, diatrizoate meglumine 7g, L-methyl sulfide Valine 0.003g, L-valine 0.011g and L-leucine 0.012g were fully dissolved in sterile water for injection to prepare a physiological solution 100mL, and then filtered through a 0.22μm filter membrane to obtain a sterile, endotoxin-free inside the container.

Embodiment 3

[0040]In an environment with local 100-level laminar flow purification conditions, at 20°C, dextran 1.5g, trehalose 5.5g, ultra-low viscosity sodium alginate 1g, diatrizoate meglumine 6g, L-formazol Thionine 0.004g, L-valine 0.009g and L-leucine 0.01g were fully dissolved in sterile water for injection to prepare a physiological solution 100mL, and then filtered through a 0.22μm filter membrane to obtain a sterile, endotoxin-free inside the container.

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Abstract

The invention discloses a composition for separating lymphocytes and a separating medium thereof, and belongs to the technical field of biology. The composition for separating single cells is prepared from the following raw materials in parts by weight: 1-4 parts of dextran, 4-6 parts of trehalose, 0.3-1.5 parts of ultralow-viscosity sodium alginate, 4-7 parts of meglumine diatrizoate, 0.003-0.006 part of L-methionine, 0.008-0.011 part of L-valine and 0.01-0.012 part of L-histidine. All raw materials in the formula are in accordance with the standard of intravenous injection grade raw materials and have small uncertainty and high safety, and the separated lymphocytes have relatively good tumor-killing activity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composition for separating lymphocytes and a separation solution thereof. Background technique [0002] As a traditional separation technique, density gradient centrifugation (Density Gradient Centrifugation) has existed for half a century for cell separation. The cell separation medium product based on the density gradient centrifugation method is the cell therapy auxiliary material. The blood sample to be separated is spread on the separation medium, and then it is centrifuged at a constant temperature at a low speed. Each cell population in the sample will be distributed in different parts of the centrifuge tube due to density differences. For example, if the separation medium has the same density as the target cell population, the target cells will be enriched at the interface between the separation medium layer and the sample layer. The layer of cells is aspirated to achieve ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0781C12N5/0783
CPCC12N5/0635C12N5/0636
Inventor 宫喜魁李若鲲马艳马艳玲郝丽敏
Owner 北京弘润天源基因生物技术有限公司
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