Efficient test cross breeding method of maize inbred line combining ability

A technology of combining ability and inbred lines, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of poor effect, waste of manpower, material resources and time, low selection accuracy, and improve selection efficiency. , saving manpower and material resources, the effect of high measurement accuracy

Inactive Publication Date: 2016-04-27
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, it is not possible to effectively select individual plants with high combining ability in the early generation panicle row of the tested line, which may cause the individual plants with high combining ability to be eliminated or lost due to field performance and other reasons.
Therefore, the selection accuracy is low and the effect is poor
Often after many years of selection, the selfing panicle line is stable, the agronomic traits are good, but the combining ability is not high, it is difficult to breed strong dominant hybrids, and has to be eliminated
Cause waste of manpower, material resources and time

Method used

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  • Efficient test cross breeding method of maize inbred line combining ability

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Experimental program
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Effect test

Embodiment 1

[0025] 1. Selection of basic materials: (Qi 319 2 ×Qi 205) BC1, using Qi 319 as the female parent, crossed with Qi 205 to produce F1; then used F1 as the female parent to backcross with Qi 319 to produce a backcross generation, namely: (Qi 319 2 × Qi 205) BC1.

[0026] 2. Early generation (S2 generation) system (tested system): (Qi 319 2 ×Qi 205) BC1, 2 generations of continuous selfing, produced (Qi 319 2 ×Qi 205) BC1S2, which is the early generation line (Qi 319 2 ×Qi 205) BC1S2 is also the tested system.

[0027] 3. Test species: choose the yellow modified line Lx9311 as the test species.

[0028] Use (Qi 319 2 ×Qi 205) BC1S2 to breed an excellent inbred line—Qi 3925, the steps are as follows:

[0029] Hainan in the winter of 1999: as figure 2 As shown, the tested system (Qi 319 2 × Qi 205) BC1S2 listing number, (Qi 319 2 × Qi 205) BC1S2-1, (Qi 319 2 × Qi 205) BC1S2-2, (Qi 319 2 ×Qi 205) BC1S2-3,..., (Qi 319 2 × Qi 205) BC1S2-10. Half of the pollen of the test...

Embodiment 2

[0049] Winter 2005: With Qi 3925 as the female parent and Huang 572 as the male parent, another hybrid Ludan 7055 was used.

[0050] In 2006, Ludan 7055 participated in the preliminary test of new maize hybrids in Sichuan Province, and its yield increased by 12% compared with the control variety Chuandan 13.

[0051] In 2007, Ludan 7055 participated in the regional test of new maize hybrids in Sichuan Province, and its average yield increased by 8.7% compared with the control variety Chuandan 13.

[0052] In 2008, Ludan 7055 participated in the regional test of new maize hybrids in Sichuan Province, and its average yield increased by 8.0% compared with the control variety Chuandan 13.

[0053] In 2009, Ludan 7055 participated in the production test of new maize hybrids in Sichuan Province, and its average yield increased by 9.7% compared with the control variety Chuandan 13.

[0054] In 2010, Ludan 7055 was approved by the Sichuan Provincial Crop Variety Committee, named Luda...

Embodiment 3

[0056] Winter 2010: With Qi 3925 as the female parent and Luxi 032 as the male parent, another hybrid Ludan 7091 was used.

[0057] In 2011, Ludan 7091 participated in the joint identification of new varieties in Anhui Province.

[0058] From 2012 to 2013, he participated in the regional examination in Anhui Province.

[0059] Participated in the production test in Anhui Province in 2014.

[0060] It was approved in 2015 and named Tianyiqing 7096.

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Abstract

The invention provides an efficient test cross breeding method of maize inbred line combining ability. The method includes: adopting a plant test cross method, only retaining single plants with high combining ability, growing next generations in head progeny rows, and then performing plant test cross till the head progeny rows are stable; secondly, according to a conventional breeding procedure, matching cross combination, performing multi-site test, regional test and the like, and finally breeding a new crossbreed to finish the whole breeding procedure. Compared with the conventional technique, the method has the advantages that the inbred line combining ability can be maintained at a high level, measurement accuracy is high, and efficiency of breeding the inbred lines with high combining ability can be increased by more than 50%. The problem of blindness in combining-ability test cross of the conventional technique is solved, accuracy in breeding the inbred lines with high combining ability is improved, and a foundation is laid for improving efficiency of commercialized maize breeding.

Description

technical field [0001] The invention relates to the field of crop breeding, in particular to a high-efficiency test cross breeding method for combining ability of maize inbred lines. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The main way to increase maize yield is to use maize heterosis. The focus of maize breeding work is to develop maize hybrids. Breeding maize inbred lines is the basis for cultivating maize hybrids; through crossing between inbred lines to prepare maize combinations and breed new maize hybrids. Therefore, the technology of breeding maize inbred lines is the core technology of maize breeding. The traditional breeding technology of maize inbred lines requires years of multi-generational self-selection. After the inbred lines are stable, they are then crossed with test species to prepare test-cross species, and the combining ability is determined, and then excellent inbred lines are selected. and hybrids. However, w...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 刘治先孙浩洋杨菲
Owner MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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