Sea cucumber polysaccharide and sea cucumber collagen polypeptide combined preparation method

Abstract

The invention provides a sea cucumber polysaccharide and sea cucumber collagen polypeptide combined preparation method. The sea cucumber polysaccharide and sea cucumber collagen polypeptide combined preparation method includes subjecting Acaudina Molpadioides serving as a raw material to mild two-stage enzymolysis treatment and obtaining Acaudina Molpadioides polypeptide mixed liquor and Acaudina Molpadioides polysaccharide mixed liquor by an ultrafiltration membrane separation technology; selecting ultrafiltration membranes different in molecular weight cut-off to achieve graded separation of Acaudina Molpadioides polypeptide and Acaudina Molpadioides polysaccharide so as to obtain Acaudina Molpadioides polypeptide products with three molecular weights and Acaudina Molpadioides polysaccharide products with four molecular weights. The sea cucumber polysaccharide and sea cucumber collagen polypeptide combined preparation method has the advantages that sea cucumber polypeptide and sea cucumber polysaccharide products different in molecular weight in Acaudina Molpadioides can be prepared in a combined manner, so that waste discharge during extraction is reduced and production cost is lowered.

Description

technical field [0001] The invention relates to a joint preparation method of sea cucumber polysaccharide and sea cucumber collagen polypeptide, which belongs to the technical field of separation and purification. Background technique [0002] There are about 140 kinds of sea cucumbers (seacucumber, holothurians) in my country, of which there are about 40 kinds of edible ones. Sea cucumber, rough sea cucumber, square pillar pentagonal melon, sea sweet potato, etc. Haitian melon, commonly known as sea eggplant, is one of more than 20 kinds of edible sea cucumbers in my country, but its edible quality is poor. [0003] The body wall is the main food (medicine) part of sea cucumbers. The research on sea cucumbers by domestic and foreign scholars has found that the body walls of sea cucumbers are very rich in nutrients. The organic components of the body walls of dried sea cucumbers contain nearly 90% protein, which is rich in amino acids needed by the human body. At the same t...

AI Technical Summary

Problems solved by technology

[0006] At present, there are chemical methods to hydrolyze sea cucumber proteins, and some use enzymatic hydrolysis methods to extract individual sea cucumber polypeptides or sea cucumber polysaccharides through a one-step hydrolysis method. The above methods can only obtain one active substance of sea cucumbers (or sea cucumber polypeptides or sea cucumber polysaccharides). Other effective sea cucumber active substances are wasted during extraction, and the obtained sea cucumber polypeptides are substances with a variety of molecular weights mixed, because active sea cucumber peptides are usually small molecular peptides such as dipeptides and tripeptides, and the large molecular weight ones Sea cucumber peptides are not conducive to absorption; in addition, chemical hydrolysis of sea cucumber proteins is prone to produce toxic substances while extracting active substances of sea cucumbers

At the same time, there are many research reports on the extraction methods of sea cucumber polysaccharides, but there are few reports on the method of jointly preparing sea cucumber polysaccharides and sea cucumber protein polypeptides with several molecular weights using low-value sea cucumbers.