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A method for preparing fucoidan and fucoxanthin by enzymatic method

A technology of fucoxanthin and fucoidan, applied in the directions of organic chemistry, fermentation, etc., can solve the problems of low efficiency, need to be improved, waste of resources, etc., and achieve the effect of simple operation

Active Publication Date: 2015-08-19
福建九为生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current technology used to prepare brown algae active substances is mainly aimed at a certain active substance. For example, while extracting fucoidan, the preparation of fucoxanthin is not taken into account, which causes a great waste of resources.
In addition, because fucoidan is wrapped by the fibrous tissue of the cell wall, and fucoxanthin is distributed in brown algae cells, the extraction efficiency of fucoidan and fucoxanthin by conventional techniques is low
[0004] Therefore, the current process still needs to be improved

Method used

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  • A method for preparing fucoidan and fucoxanthin by enzymatic method
  • A method for preparing fucoidan and fucoxanthin by enzymatic method
  • A method for preparing fucoidan and fucoxanthin by enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation of fucoidan and fucoxanthin from dried kelp

[0041] (1) Take 100 Kg of dried kelp, remove sand and sundries, dry, pulverize, and sieve through 80 meshes to make kelp dry powder;

[0042] (2) Take 50 Kg of the above dry kelp powder and mix well with 25 g of compound enzyme, add 750 L of deionized water, and stir evenly. After reacting for 5 h at room temperature at 20 °C, centrifuge in a tubular continuous centrifuge (10,000 rpm for 10 minutes) to obtain about 400 Kg of supernatant and 400 Kg of kelp residue. In this step, the brown algae is treated with biological enzymes to break the wall, so that the fucoidan and fucoxanthin in the brown algae can be fully dissolved, thereby significantly improving the extraction efficiency. The damage effect of biological enzymes on the cell wall of brown algae was observed by optical microscope (see figure 1 ). Depend on figure 1 It can be seen that before the enzyme treatment, the cells of the brown al...

Embodiment 2

[0051] Example 2: Preparation of fucoidan and fucoxanthin from dried wakame

[0052] (1) Take 100 Kg of dried wakame, remove sand and sundries, dry, pulverize, and sieve through 80 meshes to make dry wakame powder;

[0053] (2) Mix 50 Kg of the above dry wakame powder with 100 g of compound enzyme, add 1000 L of deionized water, and mix well. After reacting for 7 hours at room temperature at 20°C, centrifuge in a tubular continuous centrifuge (10,000 rpm for 10 minutes) to obtain about 600 Kg of supernatant and 450 Kg of wakame residue;

[0054] (3) Add 900 L of deionized water to the above wakame residue, extract and centrifuge under the above conditions, the extraction time is 2 hours, and the supernatant liquid is about 900 Kg, and the wakame residue is about 300 Kg;

[0055] (4) The two supernatants were combined, concentrated hydrochloric acid (HCl) was adjusted to a concentration of 4 mol / L, and added to the supernatant in proportion to make the HCl concentration reach...

Embodiment 3

[0061] Example 3: Preparation of Fucoidan and Fucoxanthin from Dried Hijiki

[0062] (1) Take 100 Kg of dried hijiki, remove sand and sundries, dry, pulverize, and sieve through 80 meshes to make hijiki dry powder;

[0063] (2) Take 50 Kg of the above-mentioned hijiki dry powder and 5 g of compound enzyme and mix well, add 1250 L of deionized water, and stir evenly. After reacting for 8 hours at room temperature at 20°C, centrifuge in a tubular continuous centrifuge (10,000 rpm, 10 minutes) to obtain about 920 Kg of supernatant and 380 Kg of hijiki dregs;

[0064] (3) Add 800 L of deionized water to the above-mentioned hijiki dregs, extract and centrifuge under the above conditions, the extraction time is 2 hours, and the supernatant liquid is about 900 Kg, and the hijiki dregs are about 280 Kg;

[0065] (4) The two supernatants were combined, concentrated hydrochloric acid (HCl) was adjusted to a concentration of 4 mol / L, and added to the supernatant in proportion to make t...

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Abstract

The invention provides a method for preparing algae fucosan and fucoxanthin in an enzymic way. The method specifically comprises the following steps of: preparing brown algae dry powder; processing the brown algae dry powder by enzymolysis: uniformly mixing the brown algae dry powder with a complex enzyme preparation, then adding deionized water to perform enzymolysis; performing acid treatment; acquiring concentrated liquor; and acquiring fucosan: acquiring crude fucoxanthin, and then acquiring pure fucoxanthin. The complex enzyme is composed of pectinase, hemicellulase and cellulose. The brown algae fucosan and the fucoxanthin prepared by the method are high in yield. The method is simple in operation, environment-friendly, efficient, and suitable for being used for synchronously extracting high-purity fucosan and fucoxanthin from brown algae with massive operation.

Description

technical field [0001] The invention relates to the field of preparation technology of fucoidan and fucoxanthin, in particular to a method for enzymatically preparing fucoidan and fucoxanthin from brown algae. Background technique [0002] Brown algae are rich in functional active substances such as active polysaccharides and carotenoids, and are a treasure house of resources for the development of drugs and functional foods. However, for a long time, brown algae (such as kelp) have only been consumed as raw materials for fresh food, low-grade processed foods or industrial products such as agar, alginate, etc., and the potential economic value of brown algae is far from being fully tapped. Modern medical and biological studies have shown that brown algae are rich in functional active substances such as marine sulfated polysaccharides (fucoidan) (3-4%) and fucoxanthin (0.5%), which play a role in liver protection, anti-virus, anti- It has shown good efficacy in biological fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04C12P19/14C07D303/32C12P17/02
Inventor 刘翼翔吴永沛闫相勇
Owner 福建九为生物技术有限公司
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