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A multi-mode scanning device of a two-photon fluorescence microscope

A two-photon fluorescence and scanning device technology, applied in the field of microscopy, can solve the problem of single structure of the galvanometer scanning device, inconvenience for users to switch between full scanning and partial scanning, etc., achieve high scanning frequency, save cost, and facilitate light stimulation The effect of the experiment

Active Publication Date: 2016-04-27
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0006] However, the structure of the traditional galvanometer scanning device is simplified, and a single galvanometer-type galvanometer or resonance-type galvanometer is used, which is not convenient for users to switch between full scanning and partial scanning.

Method used

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  • A multi-mode scanning device of a two-photon fluorescence microscope
  • A multi-mode scanning device of a two-photon fluorescence microscope
  • A multi-mode scanning device of a two-photon fluorescence microscope

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Embodiment Construction

[0039] Preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0040] In order to achieve the purpose of the present invention, in some embodiments of a multi-modal scanning device for a two-photon fluorescence microscope,

[0041] Such as Figure 1-3 As shown, a multi-modal scanning device for two-photon fluorescence microscopy includes:

[0042] The first laser 1 is used to emit a first laser source. In an embodiment, the first laser is a femtosecond pulse laser with a wavelength of 850nm;

[0043] a dichroic mirror 2, the first laser source is transmitted through the dichroic mirror 2;

[0044] The first incident light path includes: a first galvanometer type vibrating mirror 11, a first scanning lens 10 and a first tube lens 9;

[0045] The second incident optical path includes: a resonant vibrating mirror 5, a second scanning lens 6 and a second barrel lens 7;

[0046] The outgoing optical path ...

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Abstract

A multi-mode scanning device of a two-photon fluorescence microscope is disclosed. The scanning device comprises a first laser used for emitting a first laser source, a first incident light path comprising a first galvanometer scanner, a second incident light path comprising a resonance-type scanner, an emergent light path comprising a second galvanometer scanner and a micro objective, a first rotatable reflector used for communicating the first laser with the first incident light path or the second incident light path, and a second rotatable reflector used for communicating the emergent light path with the first incident light path or the second incident light path. The first laser source is adopted as incident light and switched and selected through the first rotatable reflector and the second rotatable reflector so as to allow the incident light to enter the emergent light path through the first incident light path or through the second incident light path. The scanning device is low in cost, high in efficiency and high in precision and facilitates real-time switching between global scanning and local scanning by a user.

Description

technical field [0001] The invention belongs to the technical field of microscopes, and in particular relates to a multi-mode scanning device for a two-photon fluorescence microscope. Background technique [0002] Two-photon fluorescence microscopy imaging technology uses near-infrared femtosecond laser as a light source, which can perform biomicroscopic imaging of thicker biological tissues. It has the characteristics of large imaging depth, small optical damage, high spatial resolution and contrast. The photostimulation experiment of another wavelength laser can be used to study the movement characteristics and molecular dynamics of proteins in living cells. [0003] The scanning system can generally be divided into two types: one method is based on a nanoscale displacement platform, which scans the sample by directly moving the sample fixed on the sample stage, and the other method uses a galvanometer to scan, using a scanning lens and The tube lens realizes the scanning...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01G02B21/00
CPCG01N21/01G01N21/64G02B21/00
Inventor 桂征宇蔡夫鸿娄艳阳李辉
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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