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Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes

A kind of lotus petal orchid root and direct technology, applied in the field of hybrid lotus petal orchid rhizome direct induction into seedlings, can solve the problems such as rhizome induction into seedlings that have not been reported, and achieve shorten induction time, reduce production cost, expand The effect of reproduction coefficient

Inactive Publication Date: 2016-05-04
YUNNAN YESHENGLAN FAVORITES BASE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination of Cmbidium plant seeds has been studied, but the hybridization, aseptic germination and rhizome induction of the above Cmbidium plants have not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The invention discloses a method for directly inducing seedling formation from rhizomes of hybrid lotus petal orchids. The method for inducing seedling formation includes the following steps:

[0018] Screening of material sources: Herbs and Dianhelan strains are collected in the wild, cultivated in the resource garden until the fruit reaches nine maturity, and the fruit is picked when the peel is slightly yellow;

[0019] Treatment of explants: Disinfect the above-mentioned nine-ripe fruit with 75% alcohol for 30 seconds, transfer to 0.1% mercuric chloride for surface disinfection for 8 minutes, rinse with sterile water for 3 times, take out the seeds in the sterile room and wrap the seeds with gauze, and dry them with sterile water. Rinse with bacterial water for 5 times, then pretreat with 0.1mol / L KOH solution for 8 minutes, rinse with sterile water for 3 times, disinfect with 20% sodium hypochlorite for 15 minutes, rinse with sterile water for 5 times, inoculate int...

Embodiment 2

[0023] The invention discloses a method for directly inducing seedling formation from rhizomes of hybrid lotus petal orchids. The method for inducing seedling formation includes the following steps:

[0024] Screening of material sources: Herbs and Dianhelan strains are collected in the wild, cultivated in the resource garden until the fruit reaches nine maturity, and the fruit is picked when the peel is slightly yellow;

[0025] Treatment of explants: Disinfect the above-mentioned nine-ripe fruit with 75% alcohol for 60 seconds, transfer to 0.1% mercuric chloride for surface disinfection for 12 minutes, rinse with sterile water for 4 times, take out the seeds in the sterile room and wrap the seeds with gauze, and dry them with sterile water. Rinse with bacterial water for 5 times, then pretreat with 0.1mol / L KOH solution for 10 minutes, rinse with sterile water for 3 times, disinfect with 20% sodium hypochlorite for 20 minutes, rinse with sterile water for 5 times, inoculate i...

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PUM

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Abstract

The invention relates to the technical field of plant culture in vitro and discloses a direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes. The direct induction seedling development method includes the following steps of material source screening, explant treatment, seed culture and seedling development induction culture. The problems that traditional cymbidium breeding mainly aims at division propagation, the division propagation coefficient is low, and large-scale production is difficult to achieve are solved. By means of a tissue culture method, the cymbidium propagation coefficient can be increased, a large number of test tube seedlings can be produced within a short period, and the industrial production requirement of cymbidium is met. Culture parameters and steps are verified through experiments, multiple root induction seedling development steps in traditional terrestrial orchid tissue culture are optimized, the rhizomes are induced into sprouts and roots at a time to form newborn plants, the induction process is reduced, induction time is shortened, production cost is remarkably reduced, and a corresponding culture method and technological parameters are disclosed.

Description

technical field [0001] The invention relates to the technical field of plant in vitro cultivation, in particular to a method for cultivating seedlings at one time after aseptic germination of the seeds of the lotus petal orchid plant to form a rhizome, in particular to a hybrid rhizome of the lotus petal orchid directly induced into a seedling seedling method. Background technique [0002] Medicinal herbs and Dianhe are both excellent varieties of Orchid orchids. They have strong plants, fragrant flowers and high ornamental value. They are important orchid germplasm resources. The two orchid varieties are mostly produced in northwestern Yunnan, southern Sichuan and the adjacent areas of these two places. They are good potted flower materials and hybrid parents. [0003] Orchid seeds are small and contain some incompletely developed spherical embryos without endosperm. It is difficult to germinate in a natural state. It needs to be symbiotic with orchid bacteria or cultured ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘忠贵徐锐仙刘乘里李枝林
Owner YUNNAN YESHENGLAN FAVORITES BASE CO LTD