Method for preparing low-sodium nutritional salt substitute with liquid state fermentation technology
A technology of liquid fermentation and sodium nutrition is applied in the field of preparing low-sodium nutritional substitute salt, which can solve the problems of high cost and lack of raw materials, and achieve the effects of short production cycle, stable product quality and wide sources.
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Embodiment 1
[0039] 1) Inoculate yeast strains, shiitake mushroom strains and Cordyceps militaris strains on PDA medium for slant activation for 6 days, and place them under light conditions for 4 days;
[0040] 2) Seed culture: inoculate the activated strain in step 1) into a fermenter with 1.5L of seed culture medium, set an air volume of 1:0.6, rotate at a speed of 300 rpm, and cultivate at a temperature of 28°C for 11 hours to obtain a seed culture solution;
[0041] 3) Fermentation tank culture: inoculate the seed culture solution obtained in step 2) in a 2.5L fermenter with a fermentation medium in a 10% inoculum size, with a liquid filling volume of 60%, a stirring speed of 260r / min, and an air flow of 0.8 L / min, cultivated at 28°C for 48 hours, and added 30% glucose during the cultivation process to obtain the fermentation medium;
[0042] 4) Stir the fermentation culture solution obtained in step 3) with 2 times the amount of water, heat at 100°C for 80 minutes, cool down to 45°C an...
Embodiment 2
[0046] 1) Inoculate yeast strains, shiitake mushroom strains and Cordyceps militaris strains on PDA medium for slant activation for 6 days, respectively, and place them under light conditions for 3 days of cultivation;
[0047] 2) Seed culture: inoculate the activated strain in step 1) into a fermenter with 1.5L of seed culture medium, set an air volume of 1:0.6, rotate at 300 rpm, and cultivate at a temperature of 28°C for 12 hours to obtain a seed culture solution;
[0048] 3) Fermentation tank culture: inoculate the seed culture solution obtained in step 2) into a 2.5L fermenter with a fermentation medium in a 10% inoculum size, with a liquid filling volume of 60%, a stirring speed of 260r / min, and an air flow of 1.2 L / min, cultivated at 28°C for 48 hours, and added 30% glucose during the cultivation process to obtain the fermentation medium;
[0049] 4) Stir the fermentation culture solution obtained in step 3) with 2 times the amount of water, heat at 100°C for 120 minute...
Embodiment 3
[0053] 1) Inoculate yeast strains, shiitake mushroom strains and Cordyceps militaris strains on PDA medium for slant activation for 6 days, respectively, and place them under light conditions for 3 days of cultivation;
[0054] 2) Seed culture: Inoculate the activated strain in step 1) into a fermenter with 1.5L of seed culture medium, set an air volume of 1:0.6, rotate at a speed of 300 rpm, and cultivate at a temperature of 28°C for 10 hours to obtain a seed culture solution;
[0055] 3) Fermentation tank culture: inoculate the seed culture solution obtained in step 2) into a 2.5L fermenter with a fermentation medium in a 10% inoculum size, with a liquid filling volume of 60%, a stirring speed of 260r / min, and an air flow of 0.4 L / min, cultivated at 28°C for 48 hours, and added 30% glucose during the cultivation process to obtain the fermentation medium;
[0056] 4) Stir the fermentation culture solution obtained in step 3) with 2 times the amount of water, heat at 100°C for...
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