Method for obtaining sterile mammal with rapidness and high efficiency
A mammal and the obtained technology are applied in animal husbandry and other fields, which can solve the problems of low survival rate, large population, long time interval, etc.
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Embodiment 1
[0060] Example 1. Acquisition of germ-free mice
[0061] 1. Sperm collection and sperm capacitation in male mice
[0062] Male rats of the right age to be purified (provided by Shanghai Slack Experimental Animal Co., Ltd., production license: SCXK (Shanghai) 2012-0002; use license: SYXK (Shanghai) 2012-0002), above 10 weeks, 1-2 Rats were sacrificed by cervical dislocation, the epididymis was separated on a clean operating table, sperm were picked out in sterile mineral oil, put into the HTF culture droplet, and the incubator was capacitated for 1.5 hours;
[0063] Composition of HTF medium: see the table below:
[0064] Drug Name
source
product number
mM
mg / 100ml
NaCl
Sigma
S5886
101.6
593.8
KCl
Sigma
P5405
4.69
35
MgSO 4 .7H 2 o
Sigma
P7774
0.2
4.9
K H 2 PO 4
Sigma
P5655
0.37
5.4
CaCl 2
Sigma
C5670
2.04
57
NaHCO 3
...
Embodiment 2
[0079] Example 2. Acquisition of germ-free mice
[0080] Under 36 ℃-37 ℃ temperature and 55%-65% humidity, repeat embodiment 1 to 86 female mice, obtain altogether 40 pseudopregnant female mice; is 100%.
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