Method for obtaining sterile mammal with rapidness and high efficiency

A mammal and the obtained technology are applied in animal husbandry and other fields, which can solve the problems of low survival rate, large population, long time interval, etc.

Active Publication Date: 2016-05-11
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this traditional caesarean method are: 1. When large-scale pollution occurs, a larger population cannot be recovered as soon as possible, and a long time interval is required; 2. Caesarean is likely to cause the death of offspring during the caesarean process , mainly because the lungs of the pups cannot open in time to breathe spontaneously; 3. The pups born by cesarean section are easy to be eaten by nursing mothers, because the natural maternal nature of nursing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Acquisition of germ-free mice

[0061] 1. Sperm collection and sperm capacitation in male mice

[0062] Male rats of the right age to be purified (provided by Shanghai Slack Experimental Animal Co., Ltd., production license: SCXK (Shanghai) 2012-0002; use license: SYXK (Shanghai) 2012-0002), above 10 weeks, 1-2 Rats were sacrificed by cervical dislocation, the epididymis was separated on a clean operating table, sperm were picked out in sterile mineral oil, put into the HTF culture droplet, and the incubator was capacitated for 1.5 hours;

[0063] Composition of HTF medium: see the table below:

[0064] Drug Name

source

product number

mM

mg / 100ml

NaCl

Sigma

S5886

101.6

593.8

KCl

Sigma

P5405

4.69

35

MgSO 4 .7H 2 o

Sigma

P7774

0.2

4.9

K H 2 PO 4

Sigma

P5655

0.37

5.4

CaCl 2

Sigma

C5670

2.04

57

NaHCO 3

...

Embodiment 2

[0079] Example 2. Acquisition of germ-free mice

[0080] Under 36 ℃-37 ℃ temperature and 55%-65% humidity, repeat embodiment 1 to 86 female mice, obtain altogether 40 pseudopregnant female mice; is 100%.

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PUM

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Abstract

The invention provides a method for obtaining a sterile mammal with rapidness and high efficiency. The method comprises the following steps: a) obtaining a sperm of a male mammal, and subjecting the obtained sperm to capacitation; b) obtaining an ovum of a female mammal; c) preparing an oosperm by utilizing the sperm obtained in the step a) and the ovum obtained in the step b); d) obtaining an aspermous male mammal; e) preparing a pseudopregnant female mammal; f) preparing an embryo by utilizing the oosperm obtained in the step c); g) obtaining a pseudopregnant female mammal containing the embryo obtained in the step f); and h) subjecting the female mammal obtained in the step g) to sterile culture so as to obtain the sterile mammal. The method provided by the invention has the advantages of short time, fast action, high efficiency and small breeding population, so the sterile mammal used for various purposes can be provided with rapidness and high efficiency.

Description

technical field [0001] The present invention relates to the field of experimental animal science. Specifically, the present invention relates to a method for rapidly and efficiently obtaining a sterile mammal and the use of the obtained sterile mammal. Background technique [0002] With the rapid development of medicine and biology, experimental zoology, which is the supporting condition of life sciences, has also received unprecedented attention. Not only has the research content of experimental zoology been further expanded and deepened, but also the demand for high-level experimental animals is also increasing. More and more, the requirements are getting stricter. From the cancellation of ordinary-level experimental animals, to clean-level animals, there is a large demand for Specificpathogen-free animals (SPF), and then to sterile animals including experimental animals with various known microorganisms (gnotal animals), for The refinement, quantification, and stable re...

Claims

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Application Information

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IPC IPC(8): A01K67/02
Inventor 刘丽均徐平郁丽丽李淼张艺宝强苏静
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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