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A kind of trichoderma dark green strain and application thereof

A technology of Trichoderma dark green, Trichoderma dark green, applied in the application, fungi, microorganisms and other directions, can solve the problem that has not yet been screened to degrade pectin and cellulose functional strains at the same time, so as to improve the quality of smoking and improve the quality of tobacco leaves. , the effect of improving the coordination of chemical components

Active Publication Date: 2019-08-23
CHINA TOBACCO GUANGXI IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic research mainly focuses on the screening of strains that degrade pectin or cellulose alone, and there is no report on the screening of strains that degrade pectin and cellulose at the same time.

Method used

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  • A kind of trichoderma dark green strain and application thereof
  • A kind of trichoderma dark green strain and application thereof
  • A kind of trichoderma dark green strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1) Streak inoculation of Trichoderma dark virides C9-13 on potato dextrose agar medium, culture at 28°C for 72 hours, then add sterile water and shake to obtain spore suspension, the concentration of spores is 10 6 a / mL;

[0033] Step 2) Inoculate the spore suspension in the fermentation medium with 10% inoculum size, cultivate it on a constant temperature shaker for 72 hours, the shaker speed is 170r / min, and the temperature is 25°C; then the fermentation culture is based on 6000r / min, 4 Centrifuge at ℃ for 5 minutes to obtain the fermentation supernatant;

[0034] Wherein the fermentation medium comprises 10 g of wheat straw powder, 1 g of ammonium sulfate, 0.2 g of urea, 1 g of peptone, 0.1 g of magnesium sulfate heptahydrate and 0.1 g of calcium chloride per liter of water.

[0035] Step 3) Spray 15 parts by weight of the fermentation supernatant evenly on the surface of 100 parts by weight of tobacco, then seal the tobacco with a plastic bag, and place it in ...

Embodiment 2

[0044] Step 1) Streak inoculation of Trichoderma dark viride C9-13 on potato dextrose agar medium, culture at 28°C for 48 hours, then add sterile water and shake to obtain a spore suspension;

[0045] Step 2) inoculate the spore suspension in the fermentation medium with a 30% inoculum size, cultivate it on a constant temperature shaker for 48 hours, the shaker speed is 100r / min, and the temperature is 28°C; then the fermentation culture is based on 3000r / min, 10 Centrifuge at ℃ for 10 minutes to obtain the fermentation supernatant;

[0046] The fermentation medium contains 5 g of wheat straw powder, 2.0 g of ammonium sulfate, 0.1 g of urea, 2.0 g of peptone, 0.5 g of magnesium sulfate heptahydrate and 0.3 g of calcium chloride per liter of water;

[0047] Step 3) Spray 5 parts by weight of the fermentation supernatant evenly on the surface of 100 parts by weight of tobacco, then seal the tobacco with a plastic bag, and place it in an oven at 50° C. for 6 hours. After the end...

Embodiment 3

[0056] Step 1) Streak inoculation of Trichoderma dark viride C9-13 on potato dextrose agar medium, culture at 28°C for 48-72 hours, then add sterile water and shake to obtain spore suspension;

[0057] Step 2) inoculate the spore suspension in the fermentation medium with an inoculum size of 5%, cultivate it on a constant temperature shaker for 56 hours, the shaker speed is 200r / min, and the temperature is 25°C; then the fermentation culture is based on 4500r / min, 8 Centrifuge at ℃ for 8 minutes to obtain the fermentation supernatant;

[0058] Described fermenting medium comprises wheat straw powder 30g, ammonium sulfate 0.5g, urea 0.5g, peptone 0.5g, magnesium sulfate heptahydrate 0.1g and calcium chloride 0.1g in every liter of water.

[0059] Step 3) Spray 30 parts by weight of the fermentation supernatant evenly on the surface of 100 parts by weight of tobacco, then seal the tobacco with a plastic bag, and place it in an oven at 50° C. for 6 hours. After the end, treat at...

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Abstract

The invention discloses a trichoderma atroviride strain and application thereof to tobacco. The strain is a trichoderma atroviride C9-13 strain, and the preservation number is CGMCC No.8077. The strain can degrade pectin and cellulose at the same time. A method for applying the trichoderma atroviride C9-13 strain to treating the tobacco comprises the steps that trichoderma atroviride C9-13 is firstly taken to be cultured, then a spore suspension is prepared, then the spore suspension is added to a fermentation medium to be cultured, fermented supernatant fluid is prepared, and finally the fermented supernatant fluid is sprayed to the surface of the tobacco. The content of pectin and cellulose of the treated tobacco is obviously reduced, smoking quality, especially the fragrance amount is improved to a certain degree, and the chemical ingredient harmony is also improved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and its application, and in particular relates to a trichoderma dark green strain and its application. Background technique [0002] Tobacco cell wall substances are composed of cellulose, hemicellulose, lignin, pectin and some mineral elements, which account for about 26% to 35% of the dry matter in flue-cured tobacco leaves, and play a role in maintaining cell structure and affecting the intrinsic quality of tobacco. have a greater impact. The higher the content of cell wall substances, the worse the quality of tobacco leaves. The content of reducing sugar and total soluble sugar in low-grade tobacco leaves is low, and the content of cellulose, hemicellulose, lignin and pectin is high, resulting in low-grade tobacco leaves with strong irritating taste, heavy green miscellaneous gas, spicy and astringent taste Mouth, so that the aroma of tobacco leaves cannot be revealed. The pectin and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A24B15/20C12R1/885
CPCA24B15/20C12N1/14
Inventor 蔡联合奚家勤韦建玉宋纪真田兆福孙建生郭建华牟文君
Owner CHINA TOBACCO GUANGXI IND
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