Method for detecting content of NHDC (Neohesperidin Dihydrochalcone) in mixed type feed additive

Abstract

The invention discloses a method for detecting the content of NHDC (Neohesperidin Dihydrochalcone) in a mixed type feed additive. The method comprises the following steps: detecting an NHDC standard solution of gradient concentration by using an LC (Liquid Chromatography) method, obtaining the peak area of an NHDC peak, and drawing a standard curve by using the concentration of the NHDC standard solution as the x-coordinate and the peak area as the y-coordinate; then extracting the NHDC in a sample by adopting a mobile phase, and combining ultrasonic treatment, thus obtaining a sample treating solution; detecting the sample treating solution under the same chromatographic condition of the NHDC standard solution, and converting, thus obtaining the content of the NHDC in the mixed type feed additive. According to the method for detecting the content of the NHDC in the mixed type feed additive, disclosed by the invention, the defect that the method for detecting the content of the NHDC in the mixed type feed additive is not provided at present is made up, detection can be carried out without complicated sample pretreatment, the peak detection is stable, and the method has the characteristics of sensitiveness, efficiency, precision and stability.

Description

technical field [0001] The invention belongs to the technical field of component analysis and detection in feed additives, and in particular relates to a detection method for the content of neomethylhesperidin dihydrochalcone in mixed feed additives. Background technique [0002] High-performance liquid chromatography uses a high-pressure infusion pump to pump mobile phases such as single solvents with different polarities or mixed solvents in different proportions, buffers, etc. into a chromatographic column equipped with a stationary phase, and inject the sample to be tested through the injection valve. The mobile phase is brought into the column, and after the components in the column are separated, they enter the detector in turn for detection, so as to realize the analysis of the sample. This method has become an important separation and analysis technique in the fields of chemistry, biochemistry, medicine, industry, agriculture, environmental protection, commodity insp...

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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is: how to provide a detection method for the content of neomethylhesperidin dihydrochalcone in mixed feed additives, so as to make up for the current lack of relevant The deficiency of the detection method enables the method to accurately quantify the content of neomethylhesperidin dihydrochalcone in mixed feed additives, and has the advantages of sensitive, accurate, efficient and stable detection results without complex pretreatment. features

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