Microbial insecticide and preparation method thereof

A technology for microbial pesticides and microbial strains, applied in the field of microbial pesticides and their preparation, can solve problems such as the use and improper handling of synthetic highly toxic pesticides

Inactive Publication Date: 2016-05-25
BEIJING CHENAO RUNZE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, many non-fatal incidents occur frequently due to the use and mishandling of highly toxic synthetic pesticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0094] Example one: the present invention relates to a kind of microbial insecticide production method operational route is:

[0095] first step. Cultivation of strains: According to the name and serial number of the strains, the Agricultural Microbiology Center of the China Microbiology Preservation Committee bought back 18 strains of three categories (sand tube strains); the sand tube strains were transplanted under sterile conditions Put it into a slant test tube with beef extract and peptone agar medium; observe its purity, no plaque, no bacteria, and more than 95% of smears are normal in shape; normal bacteria with good purity are subjected to single-bacteria body fluid Shaking table cultivation; expand the scale of the shaking table facility according to the required number or repeat the preparation once to meet the requirements of the next step of cultivation.

[0096] second step. Expanded cultivation of bacterial liquid: firstly mix the pure liquid of 18 bacterial s...

example 2

[0098] Example two, the three types of microorganisms involved in the present invention are fungi, actinomycetes and bacteria.

[0099] There are three types of fungi:

[0100] ACCC——30101←Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences←Institute of Microbiology, Chinese Academy of Sciences AS3.3675←Desert Control Institute in Yulin, Shaanxi Province, isolated from the larvae of the poplar moth; used to prevent corn borers, scarabs, and moths.

[0101] Paecilomyces farinosus (Dicks.) Brown powder Paecilomyces

[0102] ACCC——30190←Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences←Wu Jinwen, Beijing Forestry University; original serial number: 8601, controlling Chinese pine caterpillar.

[0103] ACCC——30677←Gift by Zhu Mingliang, Yunnan Academy of Tobacco Science; original serial number: ZML086; collection place: Yunnan; source of isolation: root-knot nematode eggs; used for biological control research.

[0104] Actinomycetes...

example 3

[0123] Example three. The finished preparation of the present invention is sprayed on the plants, enters the intestinal tract through the worm mouth, and the spore germination rate in the intestinal tract is 37% within 48 hours. Within 60-72 hours, the hyphae invade the intestinal wall, and the digestive juice enters the blood cavity, and the pH value of the digestive juice drops from 7.0 to 5.0 within 84 hours, and the mycelium protrudes from the intersegmental membrane, stigma, and sensory organ holes of the parasite, and dies The rate reached 95%.

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PUM

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Abstract

The invention relates to a microbial insecticide, which is a concentrated cenobium composed of 18 microbial strains classified into three types. Characterized by strong insecticidal ability, fast insecticidal speed and wide insecticidal range, the microbial strains respectively are: Metarhizium anisopliae, Paecilomyces farinosus, Phytophthora nicotianae, Streptomyces amilatus, Streptomyces culicidicus, Streptomyces griseolus subsp.hangzhouensis, Streptomyces calvus, Bacillus subtilis, Bacillus sphaericus, Bacillus laterosporus, Bacillus thuringiensis subsp.aisawai, Bacillus thuringiensis subsp.merrisoni, Bacillus thuringiensis subsp.galleriae, Bacillus thuringiensis subsp.Israelensis, Bacillus thuringiensis subsp.thuringiensis, Bacillus thuringiensis subsp.berliner, Bacillus thuringiensis subsp.dendrolimus, and Bacillus thuringiensis subsp.sotto.

Description

1. Technical field: [0001] The invention relates to a novel microbial insecticide and a preparation method thereof. 2. Technical background [0002] Since the 20th century, the world's population has grown rapidly, and the total demand for food has also increased. The "petrochemical agriculture" invented by people, chemical fertilizers and pesticides have helped humans obtain a large amount of food resources and promoted human development and progress. [0003] At present, there are 7.5 billion people in the world, which will reach 9 billion after 2050, and will approach the peak of 10.5 billion by the end of this century. Therefore, human beings' requirements for food will increase significantly. Man's reliance on "oil agriculture" is also increasing. [0004] According to the statistics of authoritative departments in the world, there are more than 11 million kinds of artificially synthesized chemical substances in the world at present, and the number has continued to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04A01P5/00A01N63/00
Inventor 黄海陶天申
Owner BEIJING CHENAO RUNZE TECH CO LTD
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