Optical microscope based on surface plasma resonance

Abstract

The invention discloses an optical microscope based on surface plasma resonance. The optical microscope comprises a laser generator, an incident angle adjusting module, a beam splitter, an optical microscopic amplification objective lens and a plasma resonance sensing chip all of which are arranged along a light path in sequence, and further comprises an image sensor. The incident angle adjusting module comprises a polarizing film used for transforming incident light into polarized light p. The incident light of the laser generator is incident on the plasma resonance sensing chip through the incident light adjusting module, the beam splitter and the optical microscope amplification objective lens at the surface plasma resonance angle theta to stimulate plasma surface resonance on the plasma resonance sensing chip, and the reflected light on the plasma resonance sensing chip is incident on the imaging sensor in an imaging mode through the optical microscope amplification objective lens and the beam splitter. The optical microscope has the advantages of being high in resolution ratio and free of fluorescence labeling.

Description

technical field [0001] The present invention relates to an optical microscope system based on surface plasmon resonance (SPR). Background technique [0002] For a long time, optical microscopes have played an extremely important role in the fields of life science, biological research, and material science. Compared with other high-resolution microscopes, the optical microscope has its unique advantages: for example, compared with the electron microscope, the optical microscope has the advantages of real-time and dynamic observation of living samples. This advantage alone makes the status of optical microscope in biological research unparalleled. But optical microscopy also has its own disadvantages, the biggest of which is that the resolution of optical microscopy is limited by the optical diffraction limit. The resolution of ordinary fluorescence microscope is also ~200nm, and ultra-high-resolution fluorescence microscope can realize the imaging of structures smaller than...

AI Technical Summary

Problems solved by technology

But optical microscopy also has its own disadvantages, the biggest of which is that the resolution of the optical microscope is limited by the optical diffraction limit

The resolution of ordinary fluorescence microscope is also ~200nm, while ultra-high-resolution fluorescence microscope can realize the imaging of structures smaller than 10nm, but all need to fluorescently label the sample, which may change the activity and other properties of the sample and fail to reflect the true nature of the sample. characteristic

Images