Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of extraction method of steroid hormone in penguin feces sample

A steroid hormone and extraction method technology, which is applied to the field of steroid hormone extraction from penguin feces samples, can solve the problems of high difficulty in penguin hormone levels, affecting the reliability of test results, difficulty in obtaining penguin blood, etc., and achieves high reliability of test results. , high reliability, good specificity

Inactive Publication Date: 2018-06-26
QINGDAO AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because it is difficult to obtain penguin blood, it is relatively difficult to measure penguin hormone levels by drawing blood. Moreover, this method is complicated to operate, and animals need to be caught and stabbed in the process of drawing blood, which causes more damage to animals. Large, and catching animals will cause stress to animals, so it will also have a certain impact on hormone levels, which in turn affects the reliability of test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting steroid hormones in penguin feces samples, comprising the following steps:

[0026] (1) Grind and pulverize the penguin feces stored in cryopreservation or cryopreservation on ice, put them in the insulation layer of the refrigerator at 4°C for 2 hours, and weigh 0.3g. Thawed penguin wet feces in a 15mL centrifuge tube;

[0027] (2) Add 3 mL of ethanol solution with a mass concentration of 80% to the centrifuge tube, shake for 1 min to mix the ethanol solution and penguin wet feces evenly, place the centrifuge tube in a constant temperature water bath shaker, and extract at 140 rpm and 70°C for 30 min;

[0028] (3) Put the centrifuge tube into a centrifuge at 3000rpm / min and centrifuge for 20min, take the supernatant in a 50mL centrifuge tube; add 3mL of ethanol solution with a mass concentration of 80% to the lower sediment, shake for 1min, and place the centrifuge tube in Constant temperature water bath shaker, extract at 140rpm, 80°C for 30mi...

Embodiment 2

[0032] A method for extracting steroid hormones in penguin feces samples, comprising the following steps:

[0033] (1) Grind and pulverize the penguin feces stored in cryopreservation or cryopreservation on ice, put them in the 4°C insulation layer of the refrigerator and store them for 2 hours, weigh 0.4g of thawed penguin wet feces into a 15mL centrifuge tube;

[0034] (2) Add 5 mL of ethanol solution with a mass concentration of 80% to the centrifuge tube, shake for 1 min to mix the ethanol solution and penguin wet feces evenly, place the centrifuge tube in a constant temperature water bath shaker, and extract at 140 rpm and 80°C for 15 min;

[0035] (3) Put the centrifuge tube into a centrifuge and centrifuge at 3000rpm / min for 20min, take the supernatant in a 50mL centrifuge tube; add 3mL of ethanol solution with a mass concentration of 80% to the lower sediment, shake for 1min, and let Leave it for 1-2 hours, then ultrasonically treat it for 20 minutes, then place the ce...

Embodiment 3

[0039] A method for extracting steroid hormones in penguin feces samples, comprising the following steps:

[0040] (1) Grind and pulverize the penguin feces that have been frozen or cryopreserved on ice, put them in the 4°C insulation layer of the refrigerator and store them for 1 hour, and weigh 0.5g of thawed penguin wet feces into a 15mL centrifuge tube;

[0041] (2) Add 5 mL of ethanol solution with a mass concentration of 80% to the centrifuge tube, shake for 1 min to mix the ethanol solution and penguin wet feces evenly, let stand at room temperature for 1-2 h, then ultrasonically treat for 20 min, and then place the centrifuge tube In a constant temperature water bath shaker, extract at 140rpm and 70°C for 20min;

[0042](3) Put the centrifuge tube into a centrifuge and centrifuge at 3000rpm / min for 20min, take the supernatant in a 50mL centrifuge tube; add 3mL of ethanol solution with a mass concentration of 80% to the lower sediment, shake for 1min, and let Leave it ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting steroid hormone in a penguin fecal sample, comprising the following steps: (1), weighing 0.3-0.5 g of thawed penguin wet feces, and putting in a 15m L centrifugal tube; (2), adding 3-5 Ml of ethyl alcohol solution 80% in mass concentration into the centrifugal tube, shaking and mixing well, and extracting at 70-80 DEG C for 15-30 min; (3), centrifuging the centrifugal tube for 20 min, and collecting supernate; adding 3 mL of ethyl alcohol solution 80% in mass concentration into lower precipitate, shaking, extracting for 15-30 min, centrifuging, and combining the supernates; (4) water bathing and evaporate drying the centrifugal tube holding the combined supernates; (5), adding 1 mL of methyl alcohol, shaking for dissolving and recycling, and placing at -20 DEG C for detection. The method has the advantages that steroid hormone can be extracted from penguin feces, operation is simple and accurate and detection results are highly reliable.

Description

technical field [0001] The invention relates to a method for extracting hormones, in particular to a method for extracting steroid hormones in penguin feces samples. Background technique [0002] Penguins are one of the most lovely animals on the earth. There are 18 species of penguins in the world, all of which are distributed in the southern hemisphere; there are about 8 species in the Antarctic and sub-Antarctic regions, of which 2 species breed on the coast of the Antarctic continent, and the others live on the coast of the Antarctic continent. Islands between the sub-Antarctic. Steroid hormones (Steroidhormone) are a class of fat-soluble hormones, and they are all cyclopentane polyhydrophenanthrene derivatives in structure. The molecular weight of steroid hormones is small and fat-soluble, and they can enter the target cells through diffusion or carrier transport. After entering the cells, the hormones first bind to receptors in the cytoplasm. Steroid hormones have ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 董焕声张婷玉潘庆杰
Owner QINGDAO AGRI UNIV