Method and system for determining mitochondria genome sequence information of various samples at the same time

A technology of mitochondrial genome and sequence information, which is applied in special data processing applications, instruments, electrical digital data processing, etc., can solve the problems of mitochondrial genome, and achieve the effect of low material and time costs, low requirements, and easy promotion and application

Active Publication Date: 2016-06-08
SHENZHEN HUADA GENE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, how to quickly and efficiently obtain the mitochondr

Method used

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  • Method and system for determining mitochondria genome sequence information of various samples at the same time
  • Method and system for determining mitochondria genome sequence information of various samples at the same time
  • Method and system for determining mitochondria genome sequence information of various samples at the same time

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Embodiment 1

[0089] The present embodiment has selected 49 species (mostly insects, see Table 1), including 47 genera and 42 families, most of the species are from different families, and a few are from the same family or subfamily (such as Cheilomenessexmaculata&Propyleajaponica, Letheconfusa&Mycalesismineus) Or even the same genus, the three Drosophila species of the genus Drosophila. This sampling strategy can be used to explore the impact of mixed sequencing of closely related species on subsequent analysis. The 49 samples include recently collected samples and previously preserved samples (collected in 2009 and 2010, see Table 2 for details).

[0090] refer to figure 1 According to the method for simultaneously determining the mitochondrial genome sequence information of multiple samples according to the present invention, the mitochondrial genome of each sample is determined according to the following steps:

[0091] 1. Acquisition of DNA

[0092] All multiple samples were separat...

Embodiment 2

[0113] The inventor has verified the final result of embodiment 1, and method and result are as follows:

[0114] Using the DNA of each sample obtained in Example 1 above, a total of 129 fragments from the CO1, ND1 and ND5 genes were sequenced for each species using Sanger sequencing technology, and the generated sequences were used to verify the assembled mitochondrial assembly result. As a result, no place inconsistent with the assembly results of the examples was found, and chimeras were not observed (Table 1 and Figure 5 ). Wherein the verification of 3 fruit fly assembly results has further proved the high efficiency of the assembly process of the present invention ( Figure 5 ). These three species of the same genus showed significant variability in the length of the mitochondrial genome, and D.erecta and D.melanogaster had slight interspecific differences. The verification of the low-variability interval shows that the assembly process of the present invention can ...

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Abstract

The invention discloses a method and system for determining mitochondria genome sequence information of various samples at the same time, wherein the various samples belong to different species. The method includes the following steps of: providing genome DNA of each of the various samples and mixing the genome DNA; performing library construction on the DNA mixture; performing sequencing on the DNA sequencing library; performing screening on the plurality of sequencing sequences to obtain a target sequence; performing sequence assembly on the target sequence to obtain the plurality of assembled sequences; performing morphological species taxonomy on each of the various samples to obtain morphological species taxonomy information of the various samples; performing species distribution on the assembled sequences based on the morphological species taxonomy information of the various samples and reference to a mitochondria protein gene database to determine the assembled sequence of each of the various samples; and respectively constructing a mitochondria genome of each of the samples based on the assembled sequence of each of the various samples, and determining the mitochondria genome sequence information.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and a system for simultaneously determining the mitochondrial genome sequence information of multiple samples. Background technique [0002] Mitochondria (mitochondrion) is an important organelle in eukaryotic cells. It exists in almost all kinds of eukaryotic cells and is at the center of metabolism and bioenergy conversion. In metazoans, the mitochondrial genome size of most species is about 16Kb, encoding 13 proteins, 2 rRNAs and 22 tRNAs. And its genome has fairly consistent structural features: very small and compact, the genes are arranged tightly, there is no or very little intergenic sequence, and all genes do not contain introns. [0003] Mitochondria are often used as important molecular markers for phylogenetic studies because of their unique phylogenetic history. For example, the International Barcode of Life project (theInternationalBarcodeofLife, iBOL, http: / ...

Claims

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Application Information

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IPC IPC(8): G06F19/24
Inventor 周欣唐敏刘山林苏旭谭美华
Owner SHENZHEN HUADA GENE INST
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