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Wheat anti-stripe rust gene Yr69 as well as linked SSR molecular marker and application method thereof

A molecular marker and stripe rust technology, applied in the field of plant genetics and wheat breeding, can solve the problems of long genetic distance and inaccurate markers, and achieve the effect of speeding up utilization, accurate identification and realization of diversification

Active Publication Date: 2016-06-22
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention solves the existing molecular markers and anti-stripe rust genes Yr69 The problems of long genetic distance and inaccurate markers provide a wheat stripe rust resistance gene Yr69 Linked SSR molecular markers and methods of use thereof

Method used

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  • Wheat anti-stripe rust gene Yr69 as well as linked SSR molecular marker and application method thereof
  • Wheat anti-stripe rust gene Yr69 as well as linked SSR molecular marker and application method thereof
  • Wheat anti-stripe rust gene Yr69 as well as linked SSR molecular marker and application method thereof

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Embodiment Construction

[0018] The segregating population F of offspring produced by crossing and backcrossing the disease-resistant parent CH7086 and Taichang 29 for genetic analysis of resistance 1 , F 2 、BC 1 and F 2:3 Families, constituting genetic mapping populations;

[0019] 1. Identification of resistance at adult plant stage

[0020] The test materials were planted in the field according to the following sowing method: the resistant parent CH7086, the susceptible parent Taichang 29 and Mianyang 11, and the F of CH7086×Taichang 29 and CH7086×Mianyang 11 1 Plant 1 row for each generation, F 2 Generation Planting 10 Rows, BC 1 Substitute planting 7 rows, 18 single-grain on-demand in each row; F 2:3 The family and parents were planted in 1 row each, and each row was sown with 15-20 seeds; the row length was 1.6m, and the row spacing was 0.25m; in order to ensure that the identification materials were fully infected, a row of Mingxian 169 was planted every 10 rows as a control for infection...

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Abstract

The invention relates to the fields of plant genetics and wheat breeding, in particular relates to a molecular marker for a wheat anti-stripe rust character, namely a wheat anti-strip rust gene Yr69 as well as a linked SSR molecular marker and an application method thereof, and aims at solving the problems that genetic distance between an existing molecular marker and the anti-strip rust gene Yr69 is long and marking is not accurate enough. The application method comprises the following steps: 1) amplifying by taking wheat DNA as a template and 2SA33 as a primer; 2) sequentially carrying out pre-degeneration, degeneration, annealing, extending, cycling, extending, and storing for later use; 3) carrying out electrophoresis, then mixing electrophoresis products with a loading buffer solution, then carrying out sample application, carrying out electrophoresis, and then dyeing and photographing; and 4) determining that Yr69 exists if a specific stripe is amplified. The invention has the advantages that firstly, the 2AS33 is obtained; and secondly, a dominant marker is adopted, the genetic distance is 1.9cm, the Yr69 gene is rapidly and accurately identified, powdery mildew resistance is predicted, and utilization of Yr69 is promoted.

Description

technical field [0001] The invention relates to the fields of plant genetics and wheat breeding, in particular to molecular markers of wheat stripe rust resistance traits, specifically wheat stripe rust resistance genes Yr69 Its linked SSR molecular markers and methods of use. Background technique [0002] by wheat stripe rust ( Puccinia striiformis west f.sp. tritici ( Pst )) caused by wheat stripe rust is the most important worldwide fungal disease. It has the characteristics of fast epidemic speed, wide disease area, and high degree of damage. When the disease is severe, it can reduce wheat production by more than 40%, or even fail. Rational use of disease-resistant varieties is the most economical, environmentally friendly, safe and effective way to control the disease. However, due to the high variability of wheat stripe rust and the emergence and prevalence of new pathogenic types of wheat stripe rust CYR31, CYR32 and CYR33, etc., as well as the large-scale sing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/29
Inventor 张晓军畅志坚贾举庆郭慧娟李欣詹海仙乔麟轶阎晓涛任永康张立生李晓华朱艳
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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