Rape sterile line with leaf margin holoblastically-carved marker characters and breeding method and application

A cracking and marking technology, which is applied in the fields of application, botanical equipment and methods, and plant genetic improvement, can solve the problems of difficult identification of population purity and true and false hybrids, unobvious marker traits of rapeseed, and susceptibility to environmental influences, etc. Achieve the effect of easily identifying restorer lines and sterile lines, ensuring seed purity and yield, and ensuring seed purity

Active Publication Date: 2016-06-29
江西省农业科学院作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Therefore, the technical problem to be solved by the present invention is to overcome the inconspicuous and unstable marker traits of rapeseed in the prior art, which is easily affected by the environment, and it is not easy to distinguish the purity of the population and the true and false hybrids, ther

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  • Rape sterile line with leaf margin holoblastically-carved marker characters and breeding method and application
  • Rape sterile line with leaf margin holoblastically-carved marker characters and breeding method and application
  • Rape sterile line with leaf margin holoblastically-carved marker characters and breeding method and application

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Embodiment 1

[0042] The rapeseed male sterile line breeding method described in this implementation with the leaf edge full crack mark character, it specifically comprises the following steps:

[0043] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:

[0044] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;

[0045] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and the BC was obtained by backcrossing with Brassica napus (RS013-008) AACC as the male parent 1 F backcross group;

[0046] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as the fem...

Embodiment 2

[0056] The method for breeding the rapeseed male sterile line with the full-cut mark character of leaf margin described in this implementation, specifically comprises the following steps:

[0057] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:

[0058] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;

[0059] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and the BC was obtained by backcrossing with Brassica napus (RS013-008) AACC as the male parent 1 F backcross group;

[0060] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as...

Embodiment 3

[0067] The method for breeding the rapeseed male sterile line with the full-cut mark character of leaf margin described in this implementation, specifically comprises the following steps:

[0068] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:

[0069] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;

[0070] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and the BC was obtained by backcrossing with Brassica napus (RS013-008) AACC as the male parent 1 F backcross group;

[0071] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as...

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Abstract

The invention provides a breeding method of a rape sterile line with leaf margin holoblastically-carved marker characters. A stable rape selfing line with the leaf margin holoblastically-carved marker characters obtained after distant hybrid breeding of a plant with the leaf margin holoblastically-carved marker characters and rape serves as a male parent, a rape sterile line with excellent productivity serves as a female parent, hybrid breeding is conducted to obtain the rape sterile line with the leaf margin holoblastically-carved marker characters, and the leaf margin holoblastically-carved marker characters in the obtained rape sterile line with the leaf margin holoblastically-carved marker characters have high specificity. The leaf margin holoblastically-carved marker characters are controlled by a pair of dominant major genes, and are stable, free of influences of environmental conditions, deep, obvious, easy to identify and capable of being discovered early. Generally, the characters can be identified when seedling euphyllas appear, and in the breeding process of the sterile line, it is beneficial for identifying and pulling out all the plants not subjected to leaf margin holoblastic carving, mixing with other seeds is prevented, and the purity of the seeds of the sterile line is ensured.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and in particular relates to a rapeseed sterile line with the trait of full-cracked leaf edge marks, a breeding method and application thereof. Background technique [0002] At present, more than 80% of my country's rapeseed production uses hybrid varieties. Hybrid varieties include three-line hybrids and two-line hybrids. [0003] The production of three-line hybrids includes restorer line propagation, maintainer line propagation, sterile line propagation and hybrid seed production. The specific process is: the seeds of restorer line isolation propagation, the maintainer line isolation propagation seeds; the sterile line is cross-breeding by the sterile line and the maintainer line; the hybrid for field production is obtained by the cross-breeding of the sterile line and the restorer line. [0004] The production of two-line hybrids includes: breeding of restorer lines, breeding of steri...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 汤洁戴兴临涂玉琴涂伟凤王丽钦张南峰张弢
Owner 江西省农业科学院作物研究所
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