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Separation method of chlorella species for high production of polysaccharide

A separation method and chlorella technology are applied in the field of economic microalgae research, development and application, which can solve the problems of restricting popularization and application, high production cost and low content, and achieve the effects of strong applicability, low cost and easy operation.

Active Publication Date: 2016-06-29
汪凡越
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, bioactive polysaccharides are generally secondary metabolites, the content in the body is generally low, and the production cost is high, which seriously restricts its in-depth research and further application [6-11]

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Selected sample materials: Wild fresh Dendrobium candidum (Dendrobium candidWal1.ExLindl.) is collected from Simao, Yunnan. It can be collected in Simao, Yunnan, and can also be purchased in Hangzhou Fengqi Flower and Bird Market and Hangzhou Wushan Square Flower and Bird Market from May to October every year.

[0024] Reagents and instruments: The components of the culture solution used in the present invention are domestic analytical pure. The CH-30 optical microscope was produced by OLYMPUS, Japan; the Ultrospec2000 UV-visible spectrophotometer was produced by Pharmacia, USA; the TE214S electronic balance was produced by Beijing Sartorius; the TGL-16A high-speed centrifuge was produced by Changsha Pingfan Instrument Company.

[0025] A method for isolating high-yield polysaccharide chlorella algae strains, comprising the following steps:

[0026] (1) In 1L of Basal culture medium, add 1.5ml glycerin, 2g rhamnose, 0.5g vitamin C successively, fully stir and dissolve, ...

example 2

[0038] Example 2: Select the wild Dendrobium candidum (Dendrobium candidWal1.ExLindl.) collected from Yandang Mountain, Zhejiang as a sample, and the polysaccharide content of 2 Chlorella pure species isolated from its stem by the method of the present invention is 35.7% and 36.3% respectively. %, which is at least 41.1% higher than that of Chlorella species Ch1-ZN5-M2, which currently has the highest polysaccharide content of 25.3%.

example 3

[0039] Example 3: Choose wild Dendrobium candidum (Dendrobium candidWal1.ExLindl.) collected from Wuyi Mountain, Fujian Province as a sample, and the polysaccharide content of 4 pure species of Chlorella isolated from its stem by the method of the present invention is 38.1%, 37.4% successively , 38.9% and 36.8%, at least 45.5% higher than that of Chlorella species Ch1-ZN5-M2 with the highest polysaccharide content of 25.3%.

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PUM

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Abstract

The invention relates to economic microalgae research and development application, and aims at providing a separation method of a chlorella species for high production of polysaccharide. The method comprises the following steps: adding glycerol, rhamnose and vitamin C to a Basal culture solution, so that a culture solution HPSC-M of the chlorella species for high production of the polysaccharide is obtained; and separating epiphytic algae from stems of wild dendrobium officinale, and conducting pressure screening in a high-temperature dry environment, so that the chlorella for high production of the polysaccharide is obtained. The method disclosed by the invention, compared with a conventional breeding method, has the characteristics of being simple and convenient to operate, low in cost, free from special requirements on the professional skills of operators, strong in applicability and the like; and a necessary technical method is provided for chlorella breeding.

Description

technical field [0001] The invention relates to the technology for the research, development and application of economical microalgae, in particular to a method for separating high-yield polysaccharide chlorella species. Background technique [0002] Chlorella (Chlorella) is one of the economical microalgae with wide distribution, fast growth, and the largest scale of cultivation and application in the world. Polysaccharide is one of the important bioactive components of chlorella, which has remarkable effects such as regulating the body's immune ability, anti-tumor, anti-virus and anti-aging [1～4] . However, biologically active polysaccharides are generally secondary metabolites, and their content in organisms is generally low and their production costs are high, which severely restricts their in-depth research and further application [6-11]. [Breeding of a new strain of Chlorella with high-yield polysaccharides and its growth-promoting and immune-regulating effects on Pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 汪凡越
Owner 汪凡越
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