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Bivalent inactivated vaccine for porcine circovirus type 2 and porcine parvovirus and preparation method thereof

A dual inactivated vaccine and porcine circovirus technology, applied in biochemical equipment and methods, vaccines, viruses, etc., can solve the problems of failed development of dual vaccines, difficulty in control, mutual interference, etc., and achieve convenient immunization Faster, less side effects, and improved survival rate

Inactive Publication Date: 2016-07-13
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are only reports in the literature that the injected piglets are injected twice, so it is suggested that PCV2 antigen and PPV antigen may interfere with each other when injected at the same time
Therefore, the dual vaccine of PCV2 and PPV has not been successfully developed.
In addition, PPV in domestic farms also has a certain degree of variation. The mutated PPV and PCV2 work together to make the sow reproductive disorder and piglet PWMS syndrome more difficult to control. Therefore, there is an urgent need for a single injection to prevent and treat sows Porcine circovirus type 2 and porcine parvovirus dual vaccine for reproductive failure in pigs, especially pregnant sows, and PWMS syndrome in piglets

Method used

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  • Bivalent inactivated vaccine for porcine circovirus type 2 and porcine parvovirus and preparation method thereof
  • Bivalent inactivated vaccine for porcine circovirus type 2 and porcine parvovirus and preparation method thereof
  • Bivalent inactivated vaccine for porcine circovirus type 2 and porcine parvovirus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation and inspection of the dual inactivated vaccine of embodiment 1PCV2, PPV

[0046] 1.1 Preparation of poisonous seeds for production

[0047] Preparation of PCV2SH strain: Dilute the virus seeds appropriately with the virus diluent (serum-free MEM medium), inoculate them on PK-15 cells (CCTCC, No. GDC0060) at a multiplicity of infection (M.O.I.) of 0.01, and incubate at 37°C for 30 minutes , add MEM cell maintenance solution containing 4% (v / v) calf serum and 2mmol / L D-glucosamine hydrochloride, culture at 37°C for 4 days, freeze and thaw 2 to 3 times, harvest virus, virus titer ≥ 10 6.5 TCID 50 / ml.

[0048] Preparation of PPVHN-2011 strain: Dilute the virus seeds appropriately with virus diluent (serum-free α-MEM medium), inoculate them in ST cells (CCTCC, number GDC0007) at a multiplicity of infection (M.O.I.) of 0.01, and culture them at 37°C Adsorb for 30 minutes, add MEM cell maintenance solution containing 1% (v / v) calf serum and 2mmol / L D-glucos...

Embodiment 2

[0101] Example 2 PCV2, PPV dual inactivated vaccine and the comparison of the immune effect of using two kinds of vaccines (PPV inactivated vaccine and PCV2 inactivated vaccine) to immunize piglets alone

[0102] 1. Materials

[0103] PCV2, PPV double inactivated vaccine, select the laboratory product among the embodiment 1 for use (batch number is trial Z001); PCV2 inactivated vaccine (SH strain), Pulaike Biological Engineering Co., Ltd. produces (batch number is 100903), virus The content is at least 10% before inactivation 6.5 TCID 50 / ml; PPV disease inactivated vaccine, Wuhan Zhongbo Biological Co., Ltd. (batch number is 101001), the virus content is at least 10% before inactivation 7.5 TCID 50 / ml.

[0104] 2. Design of animal experiments

[0105] 80 weaned piglets aged 21-28 days were selected and divided into 4 groups, 20 pigs in each group; each pig in the first group was injected with 2ml of PCV2 and PPV dual inactivated vaccine (batch number 001) in the neck mu...

Embodiment 3

[0111] Reproductive performance and growth of piglets after embodiment 3 PCV2, PPV dual inactivated vaccine immunization sow

[0112] 1. Materials

[0113] PCV2, PPV double inactivated vaccine, select the laboratory product among the embodiment 1 for use (lot number is trial Z001). PCV2 inactivated vaccine (SH strain), produced by Pulaike Bioengineering Co., Ltd. (batch number 100903); PPV disease inactivated vaccine, Wuhan Zhongbo Biological Co., Ltd. (batch number 101001).

[0114] 2. Design of animal experiments

[0115] Select 60 gilts aged 6-7 months and divide them into 3 groups with 20 gilts in each group. In the first group, each gilt was immunized with dual inactivated vaccine one month before mating, boosted 1 time after 14 days, and was immunized for the third time 1 month before delivery, with 2ml / head each time. In the second group, each gilt was immunized with PCV2 and PPV disease inactivated single vaccine at the same time 1 month before mating, and boosted i...

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Abstract

The invention relates to a multivalent vaccine for preventing and treating the infectious diseases of a pig, in particular to a combined vaccine for treating and preventing porcine circovirus type 2 and porcine parvovirus and a preparation method thereof. The multivalent vaccine selects and uses the porcine circovirus type 2 and the porcine parvovirus. The preparation method comprises the following steps of: culturing the porcine circovirus type 2, inactivating and concentrating; culturing the porcine parvovirus, inactivating and concentrating; mixing the two antigen components by proportion, and adding adjuvants to prepare into the vaccine. The bivalent vaccine prepared by the invention is convenient in use and is safer, and the immune effect is superior to that ofcombination of two single vaccines.

Description

[0001] This application is a patent application 201210010809.3 divisional application, the original application date is 2012 January 13 , the name of the invention is porcine circovirus type 2 and porcine parvovirus dual inactivated vaccine and its preparation method. technical field [0002] The invention relates to a dual inactivated vaccine of porcine circovirus type 2 and porcine parvovirus and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0003] Porcine circovirus (PCV) is the smallest animal virus found so far. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus, while PCV2 is a pathogenic virus. In addition to causing piglet multisystemic wasting syndrome (PostweaningMultisystemicWastingSyndrome, PMWS), PCV2 can also cause reproductive disorders in sows, causing serious damage to the pig industry. Economic losses. [0004] Porcine parvovirus (PPV) is one of the main pathogens caus...

Claims

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Application Information

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IPC IPC(8): A61K39/23A61K39/12A61P31/20A61P11/00A61P15/00
CPCA61K39/12A61K2039/552A61K2039/70C12N2750/00034C12N2750/14034
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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